Multi-zone analytical element and method using same
AssigneeEastman Kodak Company
InventorsDoyle E. Hill, Karl J. Sanford
Life of this patent
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Abstract
A multi-zone analytical element for the analysis of a proteinaceous or protein-bound substance contained in an aqueous liquid and a method of using the same are disclosed. The multi-zone element contains a spreading zone and a reagent zone in fluid contact. The reagent zone of the element contains an interactive composition. Incorporated in the spreading zone of the element is an immobilized polymeric material containing charge-bearing functional groups that, under conditions of use of the element, exhibit an electric charge opposite to that of said proteinaceous substance or the protein portion of said protein-bound substance.
Claims15
In a multi-zone analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous liquid, said element comprising
- (1) a spreading zone to receive and distribute therewithin an applied sample of said aqueous liquid, and
- (2) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to form a detectable species within said element,
- the improvement wherein said spreading zone comprises an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
An integral multilayer analytical element for the detection of a proteinaceous or protein-bound analyte in an aqueous fluid, said element comprising a radiation-transmissive support bearing a reagent layer and, superposed thereover, an isotropically porous spreading layer,
- (a) said reagent layer being in fluid contact with said spreading layer under conditions of use of said element and comprising a composition interactive with said analyte to provide a radiometrically detectable change within said element, and
- (b) said spreading layer comprising an immobile polymeric material which (a) is substantially non-interfering with respect to said interactive composition and (b) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte.
A method for the analysis of a proteinaceous or protein-bound analyte in an aqueous liquid sample, said method comprising
- (1) contacting said aqueous liquid sample together with a multi-zone analytical element having
- (a) a spreading zone to receive and distribute therewithin said sample and
- (b) a reagent zone in fluid contact with said spreading zone under conditions of use of said element, said reagent zone containing a composition interactive with said proteinaceous or protein-bound analyte to provide a detectable change in said element, said spreading zone containing an immobile polymeric material which (i) is substantially non-interfering with respect to said interactive composition and (ii) contains charge-bearing functional groups, said groups under conditions of use of said element exhibiting an electric charge opposite to that of said proteinaceous analyte or the protein portion of said protein-bound analyte;
- and
- (2) detecting, after a predetermined time, the detectable change produced in said element.
Dependencies are inferred from the claim text.