Biologically active materials attached to a ferromagnetic support
AssigneeBeecham Group Limited
InventorsPeter J. Robinson, Peter Dunnill, Malcolm D. Lilly
Life of this patent
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Abstract
Biologically active materials such as enzymes attached to a ferromagnetic support such as iron or iron oxide particles enable a magnetic separation of the supported biologically active material from a reaction medium after carrying out a biochemical reaction.
Claims19
A biochemically reactive substance which comprises an enzyme attached to a solid iron or iron oxide ferromagnetic support in particulate form with the support particles having a particle diameter of from 5 to 1000 microns.
A process for carrying out a biochemical reaction comprising the steps of:
- (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium in a reaction vessel to effect a biochemical reaction;
- (b) applying a magnetic field to the reaction medium and the enzyme attached to the particulate ferromagnetic support sufficient to retain the enzyme within the reaction vessel;
- and
- (c) removing the reaction medium after the reaction is completed from the reaction vessel, thereby effecting a separation of the enzyme from the reaction medium.
A process for carrying out a biochemical reaction comprising the steps of:
- (a) contacting an enzyme attached to a particulate iron or iron oxide ferromagnetic support, with the support particles having a particle diameter of from 5 to 1000 microns, with a reaction medium to form a reaction mixture in a reaction vessel to effect a biochemical reaction;
- (b) applying a magnetic field to the reaction mixture and stirring the reaction mixture;
- (c) discontinuing stirring of the reaction mixture after the reaction has been completed, but retaining the magnetic field while allowing the magnetized particles to flocculate and settle to the bottom of the reaction vessel;
- and
- (d) removing the enzyme from the bottom of the reaction vessel thereby effecting a separation of the enzyme from the reaction medium.
Dependencies are inferred from the claim text.