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US 4,150,105 AGranted
3-Ketosteroid antigenic conjugates, their preparation, antibodies and use
AssigneeBiological Developments, Inc.
InventorStanley J. Gross
Life of this patent
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Abstract
Antigenic 3-ketosteroid hapten-carrier conjugates are produced by activating a carbon atom in the 1,2 or 4 position of the steroid hapten and coupling to an immunogenic carrier through a linking agent. Specific antibodies are raised in animals and used in radioimmuno assays for the corresponding 3-ketosteroid hapten.
Claims9
A synthetic antigen having the following formula:
- ##STR16## where R.sub.1 is selected from the class consisting of --H, --OH, --COCH.sub.3 and --COCH.sub.2 OH;
- R.sub.2 and R.sub.3 are each selected from the class consisting of --H and --OH;
- R.sub.4 is selected from the class consisting of --CH.sub.3 and --CHO with R.sub.1 being --COCH.sub.2 OH, R.sub.2 being --H, and R.sub.3 being --OH when R.sub.4 is --CHO;
- where the 4-5 bond is selected from the class consisting of the double bond shown and a single bond, R.sub.1 being --OH, R.sub.2 and R.sub.3 being --H and R.sub.4 being --CH.sub.3 when the 4-5 bond is a single bond;
- wherein the antigen is the product of coupling a linking agent with the carrier and with a derivative of the steroid which derivative is activated for coupling to its 1,2 or 4 position, and Y represents the resultant linkage connecting the carrier to the 1,2 or 4 position of the steroid;
- the carrier is a macromolecule conferring antigenicity;
- and n is an integer not exceeding the number of available reactive coupling groups on the carrier.
4 more elements
Dependencies are inferred from the claim text.