Element for analysis of liquids

A multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising a radiation-transmissive support having superposed thereon a reagent layer, a radiation-blocking layer, and a registration layer,

• (a) said reagent layer comprising a composition including a non-diffusible material comprising a preformed, detectable moiety, said composition being interactive in the presence of liquid containing analyte to provide a diffusible species comprising the detectable moiety, and
• (b) said radiation-blocking layer, permeable to the diffusible species, comprising an opacifying agent, and
• (c) said registration layer adapted to receive said diffusible species,
• wherein the registration layer is the closest to the support of said layers and the radiation-blocking layer is interposed between the registration layer and the reagent layer, and wherein said preformed, detectable moiety can be selectively detected within the registration layer.

An integral multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising

• (a) an isotropically porous spreading layer comprising a non-fibrous material,
• (b) a reagent layer comprising a composition comprising a non-diffusable material including a preformed, detectable moiety, said composition being interactive in the presence of liquid containing said analyte to provide a diffusible product comprising the detectable moiety, and
• (c) a registration layer, permeable to the diffusible product, comprising a radiation-transmissive material,
• wherein the reagent layer is interposed between the spreading layer and the registration layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

An integral multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising

• (a) an isotropically porous spreading layer comprising a non-fibrous material,
• (b) a reagent layer comprising a composition comprising a non-diffusible material including a performed, detectable moiety, said composition being interactive in the presence of liquid containing said analyte to provide a diffusible product comprising the detectable moiety, and
• (c) a radiation-blocking layer, permeable to the diffusible product, comprising an opacifying agent,
• (d) a registration layer, permeable to the diffusible product, comprising a radiation-transmissive material,
• wherein the reagent layer is interposed between the spreading layer and the radiation-blocking layer, and the radiation-blocking layer is interposed between the reagent layer and the registration layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

A multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising,

• (a) a porous, reagent layer comprising a composition comprising a non-diffusible material including a preformed, detectable moiety, said composition being interactive in the presence of liquid containing said analyte to provide a diffusible product comprising the preformed, detectable moiety,
• (b) a radiation-blocking layer, permeable to the diffusible product, comprising an opacifying agent, and
• (c) a registration layer, permeable to the diffusible product, comprising a water-swellable, radiation-transmissive material.
• wherein the radiation-blocking layer is interposed between the reagent layer and the registration layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

A multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising a radiation-transmissive support having thereon,

• (a) a porous reagent layer comprising a composition comprising a non-diffusible material including a preformed, detectable moiety, said composition being interactive in the presence of liquid containing said analyte to provide a diffusible product comprising the preformed, detectable moiety,
• (b) a radiation-blocking layer, substantially impermeable to said non-diffusible material and permeable to said diffusible product, comprising an opacifying agent, and
• (c) a registration layer, permeable to the diffusible product, comprising a radiation-transmissive material,
• wherein the registration layer is adjacent the support and the radiation-blocking layer is interposed between the registration layer and the reagent layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

An integral multilayer analytical element for analysis of liquid containing a predetermined analyte, the element comprising a radiation-transmissive support having thereon,

• (a) an isotropically porous, non-fibrous reagent layer comprising a composition comprising a non-diffusible material including a preformed, detectable moiety, said composition being interactive in the presence of a liquid containing said analyte to provide a diffusible product comprising the preformed, detectable moiety,
• (b) a radiation-blocking layer, permeable to the diffusible product, comprising an opacifying agent, and
• (c) a registration layer, permeable to the diffusible product, comprising a radiation-transmissive material,
• wherein the registration layer is adjacent the support and the radiation-blocking layer is interposed between the reagent layer and the registration layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

A multilayer element for detecting amylase in liquids, the element comprising a radiation-transmissive support having thereon,

• (a) an isotropically porous reagent layer comprising a porous polymeric composition or particulate matter and a composition comprising a non-diffusible starch material including a preformed, detectable moiety, said composition being interactive in the presence of liquid containing amylase to provide a diffusible product comprising the preformed, detectable moiety,
• (b) a non-fibrous, radiation-blocking layer, permeable to the diffusible product, comprising an opacifying agent, and
• (c) a registration layer, permeable to the diffusible product, comprising a water-swellable, radiation-transmissive material,
• wherein the registration layer is interposed between the support and the radiation-blocking layer and the radiation-blocking layer is interposed between the reagent layer and the registration layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

An integral multilayer analytical element for detecting amylase in liquids, the element comprising a radiation-transmissive support having thereon,

• (a) an isotropically porous reagent layer comprising particulate matter, a surfactant and a non-diffusible composition comprising starch having chemically bonded thereto a preformed, detectable moiety that is a colorimetrically detectable dye moiety or a fluorescent moiety, said starch being interactive in the presence of liquid containing amylase to provide a diffusible product comprising the preformed, detectable moiety,
• (b) a non-fibrous radiation-blocking layer, impermeable to said non-diffusible composition and permeable to the diffusible product, comprising a pigment, and
• (c) a registration layer, permeable to the diffusible product, comprising a hydrophilic colloid,
• wherein the radiation-blocking layer is interposed between the registration layer and the isotropically porous reagent layer, wherein the registration layer is interposed between the support and the radiation-blocking layer, and wherein the preformed, detectable moiety can be detected selectively within the registration layer.

An integral multilayer element for detecting amylase in liquids, the element comprising a radiation-transmissive support having thereon,

• (a) an isotropically porous reagent layer comprising particulate matter, a surfactant and non-diffusible starch having a dye moiety chemically bonded thereto, said starch being interactive in the presence of liquids containing amylase to provide a diffusible product comprising the dye moiety,
• (b) a non-fibrous, radiation-blocking layer, permeable to the diffusible product, comprising titanium dioxide and gelatin, and
• (c) a non-fibrous, radiation-transmissive registration layer comprising gelatin and poly-(styrene-co-N-vinylbenzyl-N,N-dimethylbenzyl-ammonium chloride-co-divinylbenzene),
• wherein the registration layer is adjacent the support and the radiation-blocking layer is interposed between the reagent layer and the registration layer, and wherein the dye moiety can be detected selectively within the registration layer.