Support matrices for immobilized enzymes
AssigneeUop LLC
InventorsJoseph Levy, Murray C. Fusee
Life of this patent
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Abstract
Support matrices for immobilized enzymes comprise a combined organic-inorganic support consisting of a porous inorganic support containing an organic polymeric material which possesses functionalized pendent groups, said polymeric material being entrapped and also adsorbed in part in the pores of the inorganic porous support. The organic polymeric component of the matrix constitutes a copolymer prepared in situ from a first polyfunctional monomer, a low molecular weight polymer, a low molecular weight polymer hydrolysate, or a preformed polymer of natural or synthetic origin which is reacted with an excess of a second bifunctional reactive monomer.
Claims14
A process for the preparation of a support matrix for immobilization of enzymes which comprises treating an inorganic porous support material selected from the group consisting of gamma-alumina, silica, zirconia, silica-magnesia, silica-zirconia-alumina, and silica-alumina, said material possessing pore diameters of from about 100 to about 55,000 Angstroms and a surface area of from about 1 to about 500 square meters per gram, with a solution of water soluble polyamines selected from the group consisting of ethylenediamine, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentaethylenehexamine, hexamethylenediamine, and polyethyleneimine, removing unabsorbed polyamines after said treatment, contacting the treated support material with a solution containing a molar excess of a bifunctional monomeric material selected from the group consisting of glutaraldehyde and toluenediisocyanate, wherein said bifunctional monomer reacts with said polyamine material absorbed on said support, removing unreacted bifunctional monomeric material solution, and recovering the resultant inorganic-organic support matrix.
Dependencies are inferred from the claim text.