Preparative centrifugal chromatography device
OwnerShuyen Harrisoninventor
Life of this patent
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Abstract
An apparatus and method for preparative separation and collection of the components of a mixture by the use of a thin layer preparative centrifugal chromatography device in which a rotor mounted for rotation supports a thin layer of absorbent material used to separate the components of a mixture and a juxtaposed annular collection trough canted at an angle to the horizontal plane collects the separated and eluted components by means of a deflection surface canted toward the axis of rotation of the rotor at an angle of less than 90.degree. to the plane of the rotor. The chromatography device alternately consists of a series of rotors. The absorbent layer supported on the rotor is prepared by means of a chromatographic scraper device.
Claims18
A centrifugal chromatography device comprising:
- (a) a chamber;
- (b) a rotor mounted for rotation within said chamber and canted at an angle to the horizontal plane, said rotor consisting of a generally flat disc;
- (c) an absorbent medium supported on the upper surface of the rotor;
- and
- (d) an annular collection means canted at an angle to the horizontal plane and positioned within the chamber adjacent the outer edge of the rotor, said collection means having a deflection surface at the radially outer wall of the collection means which deflection surface is canted toward the axis of the rotor at an angle less than 90.degree. to the upper surface of said rotor.
A centrifugal chromatography device comprising:
- (a) a chamber;
- (b) a series of rotors axially spaced from each other and mounted for rotation about a common axis within the chamber;
- (c) an absorbent medium supported on the upper surface of each rotor;
- (d) a series of annular collection means canted at an angle to the horizontal and axially spaced from each other so as to be adjacent the outer edge of a corresponding rotor;
- and
- (e) means for removing separated material and carrier from each of the upper annular collection means individually and directing it individually to the absorbent medium near the axis of rotation of the corresponding next lower rotor, and means for removing the separated material and carrier from the lowest collecting means.
In the method of separating and collecting components of a mixture by centrifugal chromatography the steps of:
- (a) applying a mixture to be separated to the central area of a rotor mounted for rotation and subsequently applying a carrier for the mixture to the rotor;
- (b) separating the mixture by means of differential migration of the components of the mixture through an absorbent medium on the rotating rotor utilizing centrifugal force;
- (c) deflecting the eluted components down into an annular collection trough which is canted at an angle to the horizontal plane by means of a deflection surface as the eluted components are thrown off the rotating rotor;
- and
- (d) draining the collected components from the annular collection trough by the effect of gravity.
In combination, a chromatographic scraper device for the preparation of absorbent layer surfaces on chromatographic rotors, comprising:
- (a) a scraper blade;
- (b) a support pin, adjustably mounted at one end of said scraper blade;
- (c) a scraper brace plate attached to the opposite end of said scraper blade from said pin, said plate adjustably mounted on support pins and having a central bore;
- (d) a generally flat disc-shaped rotor having an axial shaft which engages the central bore of said scraper brace plate;
- said scraper blade;
- support pin and scraper brace plate temporarily affixed to said rotor such that the scraper blade can be positioned between a point at the circumferential edge of the rotor and a point at a radius near the axis of said rotor and rotated about the shaft of said rotor.
Dependencies are inferred from the claim text.