Immobilized catalytically active substance and method of preparing the same
AssigneeTanabe Seiyaku Co., Ltd.
InventorsIchiro Chibata, Tetsuya Tosa, Isao Takata
Life of this patent
Estimating the term…
Abstract
An enzyme or microorganism is entrapped within the gel matrix of a sulfated polysaccharide (said polysaccharide containing more than 10 w/w% of a sulfate moiety in its molecule) in the presence of ammonium ion, a metal ion, a water-soluble amine or a water-miscible organic solvent. The immobilized enzyme or microorganism thus obtained shows a high level of catalytic activity for a long period of time and can be used for continuous enzymatic reactions with substrates.
Claims64
An immobilized catalytically active substance which comprises a catalytically active enzyme or microorganism or a water-insoluble, hydrophilic, catalytically active carrier-bound enzyme or microorganism entrapped within the gel matrix of a sulfated polysaccharide (sulfate moiety content in the molecule:
- more than 10 w/w %) containing therein ammonium ion, a metal ion having an atomic weight greater than 24, a water-soluble organic amine or a water-miscible organic solvent.
A method of preparing an immobilized catalytically active substance which comprises the steps of mixing a catalytically active enzyme or microorganism or a water-insoluble, hydrophilic, catalytically active carrier-bound enzyme or microorganism with an aqueous solution of a sulfated polysaccharide (said sulfated polysaccharide containing more than 10 w/w % of sulfate moiety in its molecule), and then contacting the aqueous mixture with ammonium ion, a metal ion having an atomic weight greater than 24, a water-soluble organic amine or a water-miscible organic solvent to give the gel matrix of the sulfated polysaccharide having the enzyme or microorganism or carrier-bound enzyme or microorganism entrapped therein.
Dependencies are inferred from the claim text.