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US 4,120,945 AGranted
Substrate coated with receptor and labeled ligand for assays
AssigneeBecton, Dickinson and Company
InventorsSidney Gutcho, Henry McCarter
Life of this patent
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Abstract
Solid substrate is coated with a receptor, with a limited portion of the receptor being reversibly coated with a labeled form of a ligand to be assayed, whereby during an assay procedure the labeled form of the ligand is free to re-equilibrate with the receptor in competition with the ligand to be assayed.
Claims19
An article for the assay of a ligand, comprising:
- a solid, a receptor for both the ligand to be assayed and a labeled form of the ligand to be assayed on said solid, and a labeled form of the ligand to be assayed reversibly coated on at least 0.5% and no more than 25% of the surface of said receptor, whereby during an assay for the ligand, the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
In a process for the assay of a ligand, the improvement comprising:
- equilibrating a sample including the ligand to be assayed with a solid phase having a receptor applied thereto and a labeled form of the ligand reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor, whereby the labeled form of the ligand is removed from the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
In a process for preparing an article for the assay of a ligand wherein a receptor for the ligand to be assayed is coated on a tube, the improvement comprising:
- coating the labeled form of the ligand to be assayed on only the bottom portion of the receptor on the tube whereby the labeled form of the ligand is reversibly coated on at least 0.5% and no more than 25% of the surface of the receptor for re-equilibration with the receptor in competition with the ligand to be assayed.
Dependencies are inferred from the claim text.