Process for producing a peptide
Assignee(Zaidanhojin) Sagami Chemical Research Center
InventorsYoshikazu Isowa, Muneki Ohmori, Hideaki Kurita, Tetsuya Ichikawa, Masanari Sato, Kaoru Mori
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Abstract
A peptide having the formula X--A--B--Y wherein A and B are the same or different and represent an amino acid residue or a peptide residue; X represents an amino protective group and Y represents a carboxyl protective group, is prepared by reacting an amino acid or peptide having an N-terminal protective group or a salt thereof of the formula: X--A--OH with an amino acid or peptide having a C-terminal protective group or a salt thereof of the formula: H--B--Y in the presence of metalloproteinase in an aqueous solution having a pH which maintains the enzyme activity of said metalloproteinase.
Claims11
A process for producing a peptide having the formula:
- X--A--B--Y
- wherein A and B are the same or different and each represents an amino acid residue or a peptide residue, X represents an amino acid protective group, Y represents a carboxyl protective group, which comprises:
- reacting an acid component of an amino acid or peptide having an N-terminal protective group or a salt thereof which has the formula
- X--A--OH
- with an amine component of an amino acid or peptide having a C-terminal protective group or a salt thereof which has the formula
- H--B--Y
- in the presence of metalloproteinase enzyme produced from microorganisms in an aqueous solution having a pH necessary to maintain the activity of said metalloproteinase enzyme.
Dependencies are inferred from the claim text.