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US 4,112,068 AGranted
Canine lung cell strain culture systems and processes for the cultivation of viruses and vaccines therefrom
AssigneeCutter Laboratories, Inc.
InventorVictor J. Cabasso
Life of this patent
Estimating the term…
Abstract
A finite cell strain is derived from canine fetal lung tissue, the cells of which are fibroblast-like, diploid, and are free of reverse transcriptase and C-type particles. With a nutrient medium the cells provide a cell culture system useful in the cultivation of a number of viruses from which vaccines may be prepared.
Claims10
A process for the preparation of a vaccine which comprises:
- (a) maintaining a culture of diploid fibroblast-like cells of a finite cell strain derived from fetal canine lung tissue in a nutrient culture medium, said cells being free of reverse transcriptase and C-type particles, being free of adventitious viruses, being non-oncogenic, and remaining diploid throughout their passage range;
- (b) inoculating said medium with a virus selected from the group consisting of rabies virus, infectious bovine rhinotracheitis virus, canine distemper virus, type 3 parainfluenza virus and bluetongue virus;
- (c) cultivating said virus in said medium;
- and,
- (d) recovering a harvest of said virus therefrom.
2 more elements
A process for the preparation of a rabies vaccine which comprises:
- (a) maintaining a culture of diploid fibroblast-like cells of a finite cell strain in a nutrient culture medium, said cells being derived from fetal canine lung tissue and further characterized as being free of reverse transcriptase and C-type particles, free of adventitious viruses, being non-oncogenic, and remaining diploid throughout their passage range;
- (b) inoculating a first portion of said cell culture with a rabies virus;
- (c) serially passaging said virus for a number of passages sufficient to effect adaptation of said virus to said cells;
- (d) inoculating a second portion of said cell culture with said adapted rabies virus;
- (e) cultivating said adapted rabies virus;
- and,
- (f) harvesting the cultivation of said adapted rabies virus.
4 more elements
Dependencies are inferred from the claim text.