Process for preparing D-(-)-N-carbamoyl-2-(phenyl or substituted phenyl)glycines

A process for preparing D-(-)-N-carbamoyl-2-phenylglycine which comprises subjecting DL-5-phenylhydantoin to the action of an enzyme which is in the form of a cultured broth containing microorganisms, the separated cells of said microorganisms, the immobilized separated cells of said microoganisms or the immobilized separated cells of said microorganisms that have been cultured in the presence of a hydantoin compound, in an aqueous reaction medium of pH 7 to 10, said enzyme being capable of hydrolyzing 5-(phenyl or substituted phenyl)hydantoins so as to substantially produce only D-(-)forms of N-carbamoyl-2-(phenyl or substituted phenyl)-glycines, and recovering said D-carbamoyl glycines from the medium, said microorganism being one member selected from the group consisting of Aerobacter, Agrobacterium, Brevibacterium, Corynebacterium, Microbacterium, Micrococcus, Pseudomonas, Actinoplanes, Mycobacterium, Nocardia and Streptomyces.

A process for preparing D-(-)-N-carbamoyl-2-(hydroxyphenyl)glycine which comprises subjecting DL-5-(hydroxyphenyl)hydantoin to the action of an enzyme which is in the form of a cultured broth containing microorganisms, the separated cells of said microorganisms, the immobilized separated cells of said microorganisms or the immobilized separated cells of said microoganisms that have been cultured in the presence of a hydantoin compound, in an aqueous reaction medium of pH 7 to 10, said enzyme being capable of hydrolyzing 5-(phenyl or substituted phenyl)hydantoins so as to substantially produce only D-(-)forms of N-carbamoyl-2-(phenyl or substituted phenyl)-glycines, and recovering said D-carbamoyl glycines from the medium, said microorganism being one member selected from the group consising of Aerobacter, Agrobacterium, Brevibacterium, Corynebacterium, Microbacterium, Micrococcus, Pseudomonas, Actinoplanes, Mycobacterium, Nocardia and Streptomyces.

A process for preparing D-(-)-N-carbamoyl-2-(methoxyphenyl)glycine which comprises subjecting DL-5-(methoxyphenyl)hydantoin to the action of an enzyme which is in the form of a cultured broth containing microorganisms, the separated cells of said microorganisms, the immobilized separated cells of said microorganisms or the immobilized separated cells of said microorganisms that have been cultured in the presence of a hydantoin compound, in an aqueous reaction medium of pH 7 to 10, said enzyme being capable of hydrolyzing 5-(phenyl or substituted pheny)hydantoins so as to substantially produce only D-(-)forms of N-carbamoyl-2-(phenyl or substituted phenyl)-glycines, and recovering said D-carbamoyl glycines from the medium, said microorganism being one member selected from the group consisting of Aerobacter, Agrobacterium, Brevibacterium, Corynebacterium, Microbacterium, Micrococcus, Pseudomonas, Actinoplanes, Mycobacterium, Nocardia and Streptomyces.