Method of forming and depositing monomolecular layers of amphiphilic molecules on a substrate

A method for producing and depositing a monomolecular layer of amphiphilic molecules on a substrate, of the type comprising a first step of formation of said layer from a solution of said molecules in a suitable solvent by introduction of said solution on a liquid surface and by removal of the solvent, a second step of application of a predetermined surface pressure to the layer thus formed and a third step of deposition of said layer on a substrate, wherein the operation is performed on a liquid surface divided into at least first and second adjacent liquid containing compartments, at least two adjacent compartments being separated by a horizontal member which is partly immersed in said liquid and capable of rotating on its own axis, wherein the solution of said molecules is introduced into said first compartment, wherein removal of the solvent is carried out and wherein transfer of the molecules of the monomolecular layer and application of a predetermined surface pressure to the layer thus formed are carried out by transferring said layer from said first compartment to at least said second adjacent compartment by imparting rotational motion to said member which provides a separation between said compartments.