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US 4,085,203 AGranted
Process for preparing vaccine
AssigneeBurroughs Wellcome Co.
InventorsRonald Charles Telling, Roy John Passingham, Brian Lewis Kitchener, David George Hopkinson
Life of this patent
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Abstract
A cell culture system which comprises living eucaryotic cells, such as those of human or animal origin, or mycophyta dispersed within a solid carrier body or bed, which carrier consists of porous or particulate material capable of retaining the cells while allowing liquid media to pass through or to have contact with the said cells, and processes for preparing and maintaining such culture systems, particularly for the purpose of virus propagation.
Claims6
A process for preparing a vaccine which comprises:
- (a) preparing a carrier bed from a slurry of particulate material;
- (b) applying a liquid suspension of eucaryotic cells to the bed;
- (c) allowing the cells trapped within the internal cavities or spaces of the bed to grow;
- (d) circulating nutrient medium through the bed, whereby nutrients are delivered to the cells trapped within the internal cavities and metabolic and degradation products are removed in the liquid leaving the bed;
- (e) infecting the cells trapped within the internal cavities with a microorganism to which the cells are susceptible;
- (f) propagating the microorganism within the cells by circulating through the bed a medium suitable for growth of the microorganism;
- (g) collecting the medium leaving the bed containing the microorganisms which are released following disruption of the cells, whilst retaining cell debris within the bed;
- (h) inactivating the microorganism with an inactivating agent;
- and
- (i) adding the inactivated microorganism to a pharmaceutically acceptable carrier therefor.
7 more elements
Dependencies are inferred from the claim text.