Process for recovering microbial cellular proteins
AssigneeHitachi, Ltd.
InventorsMasahiko Ishida, Yoshitaka Oguri, Norio Shimizu, Tadashi Muroi
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Abstract
Proteins contained in microbial cells, for example, cells of petroleum-assimilable yeast, are separated and purified by extracting proteins from microbial cells, treating the resulting extract solution with a cation exchanger, treating said cation exchanger containing the adsorbed proteins with a volatile basic substance in the presence of water thereby to desorb or elute the adsorbed proteins, removing said basic substance dissolved in the resulting eluate by evaporation, and then concentrating the eluate thereby to obtain the dissolved proteins.
Claims35
A process for recovering with high purity substantially all of the different microbial cellular proteins having different physico-chemical properties from a solution obtained by extracting microbial cells with an aqueous extraction solution, comprising (1) contacting the solution with a strong cation exchanger at a pH value of less than 4.5 thereby adsorbing the proteins onto said cation exchanger, the cation exchanger being sufficiently swollen by water so that ions of the proteins can easily arrive at exchanging groups present in the cation exchanger, and (2) desorbing the proteins adsorbed by said strong cation exchanger by contacting said cation exchanger with a volatile basic substance in the presence of water to thereby recover substantially all of said different proteins with high purity.
A process for separating with high purity substantially all of the different microbial cellular proteins having different physico-chemical properties from a solution obtained by extracting microbial cells with an aqueous extraction solution, comprising contacting the solution with a strong cation exchanger at a pH value of less than 4.5 thereby adsorbing the proteins onto said cation exchanger, the cation exchanger being sufficiently swollen by water so that ions of the proteins can easily arrive at exchanging groups present in the cation exchanger, contacting the cation exchanger containing adsorbed proteins with a volatile basic substance in the presence of water so that the proteins are desorbed or eluted and an eluate containing the proteins is formed, and removing the volatile basic substance from said eluate by evaporation.
A process for recovering with high purity substantially all of the different microbial cellular proteins having different physico-chemical properties contained in microbial cells comprising treating the microbial cells with an aqueous extracting solution so as to dissolve the different microbial cellular proteins contained in the microbial cells into the solution;
- contacting said solution containing the dissolved microbial cellular proteins with a strong cation exchanger at a pH value of less than 4.5, said cation exchanger being sufficiently swollen by water so that ions of said proteins can easily arrive at exchanging groups present in said cation exchanger, desorbing the proteins adsorbed by said cation exchanger by contacting said cation exchanger with a volatile basic substance;
- and separating the basic substance from said proteins by evaporation.
A process for recovering with high purity substantially all the different microbial cellular proteins having different physico-chemical properties contained in microbial cells which comprises treating the microbial cells with an aqueous extracting solution so as to form a suspension of said microbial cells in said solution and cause the different microbial cellular proteins contained in the microbial cells to dissolve into said solution;
- separating said solution from said suspension so that an extract solution containing said different microbial cellular proteins therein is obtained;
- contacting the extract solution with a strong cation exchanger at a pH value of less than 4.5, said cation exchanger being sufficiently swollen by water so that ions of said proteins can easily arrive at exchanging groups present in said cation exchanger;
- desorbing the proteins adsorbed by said cation exchanger by contacting said cation exchanger with a volatile basic substance;
- and separating the basic substance from said proteins by evaporation.
A process for separating with high purity substantially all of the different microbial cellular proteins having different physico-chemical properties from a solution obtained by extracting microbial cells with an aqueous solution to form an extract solution containing salts, low molecular weight substances, and high molecular weight substances including said proteins and polysaccharides, and thereafter treating said extract solution to remove said salts and low molecular weight substances therefrom and thereby produce an extract composition comprising an aqueous solution of said proteins and polysaccharides;
- said process comprising contacting said extract composition with a strong cation exchanger at a pH value of less than 4.5 thereby adsorbing the proteins onto said cation exchanger, said cation exchanger being sufficiently swollen by water so that ions of the proteins can easily arrive at exchanging groups present in the cation exchanger, contacting the cation exchanger containing adsorbed proteins with a volatile basic substance in the presence of water so that the proteins are desorbed or eluted and an eluate containing the proteins is formed, and removing the volatile basic substance from said eluate by evaporation.
Process for recovering with high purity substantially all of different microbial cellular proteins having different physico-chemical properties contained in microbial cells which comprises treating the microbial cells with an aqueous extracting solution so as to form a suspension of said microbial cells in said solution and cause dissolution into said aqueous extracting solution of (1) salts, (2) low molecular weight substances, and (3) high molecular weight substances composed of different microbial cellular proteins and polysaccharides separating said aqueous extracting solution from said suspension to produce an extract solution containing said salts, said low molecular weight substances and said high molecular weight substances;
- contacting said extract solution with a strong cation exchanger at a pH value of less than 4.5, said cation exchanger being sufficiently swollen by water so that ions of said proteins can easily arrive at exchanging groups present in said cation exchanger;
- desorbing the proteins adsorbed by said cation exchanger by contacting said cation exchanger with a volatile basic substance;
- and separating the basic substance from said proteins by evaporation.
Dependencies are inferred from the claim text.