Back to results
US 4,072,572 AGranted
E. coli detection broth for clinical use with automated microbial analyzer
AssigneeMcDonnell Douglas Corporation
InventorsJames W. Lanham, Gregory D. Rodgers, Michael C. Meyer
Life of this patent
Estimating the term…
Abstract
A medium for the detection and identification of Escherichia coli (E. coli) in urine samples. The medium employs para-coumaric acid, saponin and 3,4-dihydroxybenzoic acid to inhibit growth of unwanted microorganisms and thus eliminate false positive test results.
Claims8
A composition for selectively identifying E. coli comprising:
- a. a source of nutrients,
- b. an indicator to show the presence of E. coli organism and,
- c. means for inhibiting the growth of other coliform-like organisms which normally give positive results in tests for E. coli comprising a combination of coumaric acid, 3,4-dihydroxybenzoic acid, and saponin.
A composition for selectively identifying E. coli comprising per liter:
- a. from about 0.05 to about 1.50 gm para-coumaric acid,
- b. from about 0.05 to about 1.50 gm 3,4-dihydroxybenzoic acid,
- c. from about 0.75 to about 1.25 gm saponin,
- d. from about 4.0 to about 6.0 gm lactose,
- e. from about 4.0 to about 6.0 gm L-arabinose,
- f. from about 4.0 to about 6.0 gm nitrogen source,
- g. from about 0.75 to about 1.25 gm yeast extract,
- h. from about 1.5 to about 4.5 gm gram positive inhibitor,
- i. from about 15 to about 35 ml biological pH indicator, and
- j. distilled water to 1 liter.
7 more elements
A composition for selectively identifying E. coli comprising per liter:
- a. about 1 gm para-coumaric acid,
- b. about 1 gm 3,4-dihydroxybenzoic acid,
- c. about 1 gm saponin,
- d. about 5 gm lactose,
- e. about 5 gm L-arabinose,
- f. about 5 gm nitrogen source,
- g. about 1 gm yeast extract,
- h. about 3 gm gram positive inhibitor,
- i. about 25 ml biological pH indicator, and
- j. distilled water to 100%.
7 more elements
Dependencies are inferred from the claim text.