Klebsiella pneumoniae and Enterobacter broth
AssigneeMcDonnell Douglas Corporation
InventorsSandra F. Gibson, Gregory D. Rodgers
Life of this patent
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Abstract
A broth medium for the detection of Klebsiella, specifically Klebsiella pneumoniae and Enterobacter in urine. The medium employs 3-(.alpha.-acetonylbenzyl)-4-hydroxycoumarin and 2,4 dinitro-phenyl hydrazone of .alpha.-Ketoglutoric acid to inhibit growth of Escherichia Coli organisms and to promote growth of Klebsiella. This medium also has a pH of about 8.0 to inhibit other organisms which grow under more neutral conditions.
Claims8
A broth medium for the detection of Klebsiella or Enterobacter comprising:
- a. a nitrogen source,
- b. a carbon source,
- c. a vitamin source,
- d. a mixture of chemical inhibitors which will inhibit the growth of Escherichia Coli while simultaneously promoting the growth of Klebsiella or Enterobacter, said E. coli inhibitors being 3-(.alpha.-acetonylbenzyl) -4-hydroxycoumarin and 2,4 dinitrophenyl hydrazone of .alpha.-Ketoglutaric acid and
- e. an indicator to indicate the presence of Klebsiella or Enterobacter, at a pH of about 8.
A broth medium for the detection of Klebsiella or Enterobacter comprising a nitrogen source, a carbon source, a vitamin source, Escherichia Coli inhibitors, and an indicator to indicate the present of Klebsiella or Enterobacter, at a pH of 8.0, comprising a mixture of sodium desoxycholate and the sodium salt of ricinoleic acid.
A broth medium for the detection of Klebsiella or Enterobacter comprising per liter of medium:
- a. about 4.5 to about 5.5 gm Gelysate,
- b. about 9.0 to about 11.0 gm Cellobiose,
- c. about 9.0 to about 11.0 gm Inositol,
- d. about 0.24 to about 0.26 gm Yeast Extract,
- e. about 9.0 to about 11.0 gm sodium desoxycholate,
- f. about 0.24 to about 0.26 gm D-Biotin,
- g. about 9.0 to about 11.0 gm Sodium Salt of Ricinoleic Acid,
- h. about 1.96 to about 2.04 gm 3-(.alpha.-Acentonylbenzyl)-4- Hydroxycoumarin,
- i. about 0.49 to about 0.51 gm 2,4 dinitro-phenyl hydrazone of .alpha.-Ketoglutaric acid, and
- j. about 13.0 to about 13.5 mg/l Brilliant Green,
- k. said medium having a pH of about 8.
Dependencies are inferred from the claim text.