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US 4,072,566 AGranted
Immobilized biologically active proteins
AssigneeCorning Glass Works
InventorMerrill Lynn
Life of this patent
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Abstract
Arylamine surface derivatives useful for the immobilization of biologically active proteins such as enzymes can be prepared by adsorbing p-phenylenediamine onto the surfaces of water-insoluble inorganic carriers having a relatively low surface isoelectric point.
Claims6
A method of bonding a biologically active protein to an inorganic support material, the method comprising the steps of:
- a. reacting a high surface area, water insoluble, inorganic support material having a surface isoelectric point less than 7.0 with a solution of p-phenylenediamine under conditions sufficient to adsorb at least a portion of the p-phenylenediamine to the surface of the support via one of the amino groups on the p-phenylenediamine, the support material having been selected from the group consisting of siliceous materials, stannic oxide, titania, manganese dioxide, and zirconia;
- b. separating the support material from the solution and washing it to remove substantially all non-adsorbed p-phenylenediamine;
- and
- c. reacting the separated support material with a solution of biologically active proteins under conditions sufficient to covalently bond at least a portion of the proteins to the support via the remaining available amino groups of the adsorbed p-phenylenediamine without significant loss of biological activity.
1 more element
Dependencies are inferred from the claim text.