Method for determining free fatty acids in blood serum using fatty acid activating enzymes
AssigneeOno Pharmaceutical Co., Ltd.
InventorsZyuro Takahashi, Chozo Hayashi
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Abstract
A method for determining free fatty acids in blood serum by subjecting them to a series of enzymatic reactions in the same reaction system using an acyl coenzyme A-synthesizing enzyme, myokinase and pyruvate kinase, and measuring the resulting pyruvic acid.
Claims12
A method for determining free fatty acids in blood serum, which comprises (A) preparing a standard curve for determining free fatty acids by subjecting known amounts of free fatty acid to (I) reaction with an acyl coenzyme A-synthesizing enzyme in the presence of adenosine triphosphate and coenzyme A, (2) subjecting the resulting adenosine monophosphate to reaction with myokinase in the presence of adenosine triphosphate, (3) subjecting the resulting adenosine diphosphate to reaction with pyruvate kinase in the presence of phosphoenolpyruvic acid, and (4) measuring the amount of the resulting pyruvic acid;
- (B) carrying out said reactions (1) through (4) in the blood serum in the absence of the coenzyme A in said reaction (I);
- (C) after step (B) carrying out said reactions (1) through (4) in the blood serum with the addition of coenzyme A in said reaction (I);
- and (D) determining amount of free fatty acids in said blood serum by subtracting the value determined in step (B) from the value determined in step (C) and comparing the result with the standard curve.
Dependencies are inferred from the claim text.