Ready made sterilized culture media and process of preparation
AssigneeThe State of Israel and Isorad Isotope and Radiation Enterprise, Ltd.
InventorsEli Eisenberg, Gideon Altmann, Bianca Bogokovsky, Mordechai Lapidot
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Abstract
A process for preparing sterile culture media in unit dosage form which comprises preparing a composition of such media of conventional composition, adjusted or augmented by adding constituents in such manner that after sterilization by ionizing radiation a sterile medium of satisfactory composition is obtained. Additional constituents added are radio protectors, increased quantities of indicators, enzymes preventing the formation of toxic degradation products, vitamins or a combination of any of these.
Claims10
A process for preparing sterile culture media in unit dosage form comprising the steps of:
- preparing culture media compositions of several different conventional constitutions, at least some of said culture media including an indicator in their conventional constitutions;
- adjusting said compositions by selectively adding radio protectors, enzymes adapted to pevent formation of toxic degradation products upon said media being subjected to ionizing irradiation, or additional quantities of indicator, said indicator being added only to those compositions of said conventional constitutions normally including an indicator;
- and
- sterilizing the culture media by means of ionizing irradiation, the amount of said added constituents being such that after said sterilization step of each of said compositions a sterile culture medium of quality sufficient to produce satisfactory bacterial growth as compared to non ionizing radiation sterilized medium is obtained.
A process for preparing a sterile culture medium in unit dosage form comprising the steps of:
- preparing a culture medium composition of conventional constitution containing a conventional amount of indicator;
- adding a further quantity of indicator of at least 50% above the conventional content thereof;
- then
- putting a desired quantity of said culture medium into a container;
- and then
- sterilizing said culture medium by means of ionizing irradiation, thereby obtaining a sterile culture medium of quality sufficient to produce satisfactory bacterial growth as compared to non ionizing radiation sterilized medium.
A process for preparing a sterile culture medium in unit dosage form comprising the steps of:
- preparing a culture medium composition of conventional constitution;
- then
- adding a radio protector;
- and then
- sterilizing said culture medium by means of ionizing irradiation, thereby obtaining a sterile culture medium of quality sufficient to produce satisfactory bacterial growth as compared to non ionizing radiation sterilized medium.
A process for preparing a sterile culture medium in unit dosage form comprising the steps of:
- preparing a culture medium composition of conventional constitution;
- then
- adding an enzyme adapted to prevent formation of toxic degradation products upon said medium being subjected to ionizing irradiation;
- and then
- sterilizing said culture medium by means of ionizing irradiation, thereby obtaining a sterile culture medium of quality sufficient to produce satisfactory bacterial growth as compared to non ionizing radiation sterilized medium.
Dependencies are inferred from the claim text.