Test composition and device for determining peroxidatively active substances and process for preparing same
AssigneeMiles Laboratories, Inc.
InventorCharles Tak Wai Lam
Life of this patent
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Abstract
A test composition is disclosed which is useful in determining the presence of a peroxidatively active substance, such as hemoglobin, in a test sample. The composition comprises an organic hydroperoxide, an indicator capable of producing a detectable response in the presence of a peroxide, and a diluent having the structure ##STR1## IN WHICH R.sub.1 and R.sub.2, same or different, are hydrogen, alkyl or alkoxy having 1 to about 6 carbon atoms, or aryl. The method comprises preparing the test composition and incorporating it with a carrier matrix.
Claims40
A process for preparing a test device for determining the presence of a peroxidatively active substance in a sample, said process comprising the steps of
- a. preparing a test composition by combining an organic hydroperoxide, an indicator capable of producing a detectable response in the presence of a peroxide, and a diluent comprising a compound having the structure ##STR3## in which R.sub.1 and R.sub.2, same or different, are hydrogen, alkyl or alkoxy having 1 to about 6 carbon atoms, or aryl, and
- b. incorporating said test composition with a carrier matrix.
A process for preparing a test device capable of determining the presence of a peroxidatively active substance in a sample comprising the steps of
- a. preparing a test composition by combining cumene hydroperoxide, o-tolidine, dimethyl sulfone, and N,N-dimethyl formamide,
- b. forming a mixture from said test composition and a suitable solvent,
- c. contacting a carrier matrix with said mixture thereby incorporating the matrix with the test composition and,
- d. drying said carrier matrix.
A test composition for determining the presence of a peroxidatively active substance in a sample, the composition comprising
- an organic hydroperoxide, an indicator capable of producing a detectable response in the presence of a peroxide, and
- a diluent comprising a compound having the structure ##STR4## in which R.sub.1 and R.sub.2, same or different are hydrogen, alkyl or alkoxy having 1 to about 6 carbon atoms or aryl.
A test composition for determining the presence of a peroxidatively active substance in a sample, the composition comprising cumene hydroperoxide, o-tolidine, dimethyl sulfone and N,N-dimethyl formamide.
A test device for determining the presence of a peroxidatively active substance in a sample, the device having a carrier matrix incorporated with a test composition comprising
- an organic hydroperoxide,
- an indicator capable of producing a detectable response in the presence of a perioxide, and
- a diluent comprising a compound having the structure ##STR5## in which R.sub.1 and R.sub.2, same or different, are hydrogen, alkyl or alkoxy having 1 to about 6 carbon atoms or aryl.
A test device for determining the presence of a peroxidatively active substance in a sample, the composition comprising cumene hydroperoxide, o-tolidine, dimethyl sulfone and N,N-dimethyl formamide.
A test device for determining the presence of a peroxidatively active substance in a sample, said device being prepared by the steps of
- a. preparing a test composition by combining an organic hydroperoxide, an indicator capable of producing a detectable response in the presence of a peroxide, and a diluent comprising a compound having the structure ##STR6## in which R.sub.1 and R.sub.2, same or different, are hydrogen, alkyl or alkoxy having 1 to about 6 carbon atoms, or aryl, and
- b. incorporating said test composition with a carrier matrix.
A test device for determining the presence of a peroxidatively active substance in a sample, said device being prepared by the steps of
- a. preparing a test composition by combining cumene hydroperoxide, o-tolidine, dimethyl sulfone, and N,N-dimethyl formamide,
- b. forming a mixture from said test composition and a suitable solvent,
- c. contacting a carrier matrix with said mixture thereby incorporating the matrix with the test composition and,
- d. drying said carrier matrix.
Dependencies are inferred from the claim text.