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US 4,070,358 AGranted
Process for the preparation of diindole alkaloids or of the acid addition salts thereof
AssigneeRichter Gedeon Vegyeszeti Gyar Rt.
InventorsKarola Jovanovics, Kalman Szasz, Csaba Lorincz, Laszlo Hermann, Eszter Dezseri
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Abstract
A process is disclosed for the separation and recovery of vinblastine and leurosine from a mixture of diindole alkaloids obtained by extraction of the plant, Vinca rosea L.
Claims6
A process for isolating vinblastine from a mixture of diindole alkaloids comprising acid addition salts of vinblastine and leurosine which comprises the steps of:
- a. dissolving the mixture in an organic solvent at least partially miscible with water;
- b. adding to the solution formed in step (a) an organic base of higher basicity than that of vinblastine or leurosine;
- c. separating the precipitated leurosine;
- d. evaporating the remaining solution to leave behind a dry residue;
- e. extracting the dry residue formed in step (d) with a hydrocarbon solvent;
- f. extracting the solution formed in step (e) with an aqueous phosphate buffer solution having a pH of 3.7 to 4.3;
- g. readjusting the aqueous phase extracted in step (f) with phosphoric acid to a pH of 3.5 to 4.1;
- h. extracting the acidic aqueous phase formed in step (g) with a chlorinated hydrocarbon solvent;
- and
- i. recovering vinblastine from the chlorinated hydrocarbon solvent.
7 more elements
A process for isolating vinblastine from a mixture of diindole alkaloids comprising acid addition salts of vinblastine and leurosine which comprises the steps of:
- a. dissolving the mixture in an organic solvent at least partially miscible with water;
- b. adding to the solution formed in step (a) an organic base selected from the group consisting of monoethylamine, diethylamine and pyridine;
- c. separating the precipitated leurosine;
- d. evaporating the remaining solution to leave behind a dry residue;
- e. extracting the dry residue formed in step (d) with a hydrocarbon solvent;
- f. extracting the solution formed in step (e) with an aqueous phosphate buffer solution having a pH of 3.7 to 4.3;
- g. readjusting the aqueous phase extracted in step (f) with phosphoric acid to a pH from 3.5 to 4.1;
- h. extracting the acidic aqueous phase formed in step (g) with a chlorinated hydrocarbon solvent;
- and
- i. recovering vinblastine from the chlorinated hydrocarbon solvent.
7 more elements
Dependencies are inferred from the claim text.