Device for measuring fractionary volumes of liquid samples
AssigneeIntertechnique
InventorJean-Michel Schmidt
Life of this patent
Estimating the term…
Abstract
A device for measuring fractions of a liquid sample comprises a casing having a vertical axis and containing a central chamber and transparent cells distributed around the chamber and permanently vented to atmosphere. Each cell is connected to the chamber by a closure cup. A capillary constriction separates each pocket from the corresponding cell. The device may be used for the preparation of antibiograms.
Claims11
A device for accurately measuring predetermined volumes of a liquid sample, comprising:
- a casing having a central axis of rotation which is kept vertical in operation, a concentric upwardly open chamber to receive said liquid sample, a plurality of test cells having transparent side walls, distributed around said chamber and spaced radially therefrom, and a plurality of radial pockets each having an inner end communicating with said chamber and to be filled by liquid flowing from said chamber and an outer end communicating with a respective one of said cells by means defining a capillary constriction,
- and displaceable closure means arranged to separate said chamber from said pockets and to retain, in each of said pockets, the liquid volumes passed by gravity from said chamber to said pockets respectively,
- wherein a vent is formed in the wall of each said cell in the upper portion thereof.
A dispensable device for testing antibiotic susceptibility of bacteria in a liquid sample, comprising:
- a casing having a vertical axis,
- an annular wall in said casing defining an upwardly open central chamber,
- a plurality of radially directed pockets formed in said casing communicating with said chamber via openings in said annular wall and angularly distributed about said chamber,
- a plurality of transparent test cells arranged in a circular row around said pockets, each provided with a venting opening in the upper portion thereof,
- means defining a capillary liquid flow constriction, connecting each said test cell to a respective one of said pockets,
- closure means arranged to substantially sealingly engage said lateral wall of said chamber and to close said openings, thereby separating said cells from said chamber,
- said cells containing a bacteria culture medium and at least some of said cells containing an antibiotic at a predetermined concentration.
A method for testing susceptibility of bacteria in a liquid sample to a plurality of different antibiotics, comprising the steps of:
- providing a rotatable casing defining an upwardly open central chamber concentric with the rotational axis of said casing, a plurality of radial pockets communicating at the inner ends thereof with said central chamber for gravity flow of liquid from said chamber into each of said pockets, the outer ends of each of said pockets communicating by means of a flow restriction with each of the plurality of vented test cells formed in the rotatable casing, some at least of said cells containing a bacteria growing medium and an antibiotic in lyophylised form,
- filling said central chamber with said liquid sample until the pockets are filled up to said constrictions by gravity flow from said chamber,
- inserting said closure means to isolate said predetermined volumes of liquid in said pockets,
- rotating said casing about said axis to create centrifugal forces sufficient to overcome said capillary forces and to cause transfer of said volumes from said pockets into said test tubes,
- incubating said bacteria for a predetermined time period, and optically determining the growth of bacteria in each said cell.
Dependencies are inferred from the claim text.