Diagnostic media
AssigneeIndiana University Foundation
InventorStanton C. Burt
Life of this patent
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Abstract
Improved diagnostic media for isolating, identifying, and classifying Enterobacteriaceae comprise an effective amount of isopropyl-beta-D-thiogalactopyranoside in order to reduce the incidence of false lactose negative determinations and to differentiate between lac inducible and lac constitutive bacteria.
Claims9
In a diagnostic medium for culturing micro-organisms comprising at least one nutrient, the improvement comprising incorporating therein an amount of isopropyl-beta-D-Thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. Coli.
An improved diagnostic medium for use in isolating, identifying, and classifying Enterobacteriaceae comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galatosidase in lactose negative strains of E. coli.
A method for classifying microorganisms comprising the steps of:
- inoculating the micro-organism onto a diagnostic medium comprising at least one nutrient and an amount of isopropyl-beta-D-thiogalactopyranoside effective to induce the production of beta galactosidase in lactose negative strains of E. coli;
- incubating te inoculated medium;
- and
- assaying the inoculated medium for beta-galactosidease.
Dependencies are inferred from the claim text.