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US 4,070,242 AGranted
Method for the preparation of prostaglandin synthetase
AssigneeMiles Laboratories, Inc.
InventorSheikh Arshad Saeed
Life of this patent
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Abstract
A method for the preparation of purified prostaglandin synthetase from bovine seminal vesicles is disclosed. The method includes the steps of homogenizing bovine seminal vesicles with a liquid medium to form an homogenate and centrifuging the homogenate at a speed such that the centrifugal force does not exceed 1,000g. The supernatant obtained, containing prostaglandin synthetase, is separated from the solids.
Claims7
A method for the preparation of prostaglandin synthetase from bovine seminal vesicles which comprises the steps of:
- homogenizing said vesicles with sufficient liquid medium to form an homogenate, subjecting said homogenate to low-speed centrifugation wherein said centrifugation is sufficient to form a solid phase and a liquid phase, and wherein said centrifugation does not exceed about 1,000g, to obtain a supernatant fraction containing prostaglandin synthetase.
Dependencies are inferred from the claim text.