Method for producing antibiotic T-42082 and antibiotic T-42082

Antibiotic T- 42082 has the following properties:

• 1. Melting point:
• 120.degree.-122.degree. C;
• 2. elemental analysis:
• Found 1 C, 62.73;
• H, 9.23;
• O, 26.01;
• 2 C, 62.74;
• H, 9.36;
• O, 27.03(%)
• 3. molecular weight:
• 859 (by osmometry):
• 4. Ultraviolet absorption spectrum;
• No characteristic absorptions;
• 5. Infrared absorption spectrum:
• The dominant absorptions (wave-numbers) measured in KBr disc method are as follows:
• 2940, 1700, 1462, 1383, 1085, 972 cm.sup.-1 ;
• 6. Thin-layer chromatography;
• silica gel, ascending method, detected as a brown spot by a spray of 10% aqueous H.sub.2 SO.sub.4 ;
• ______________________________________ Solvent System Rf ______________________________________ Ethyl acetate-benzene (1:1) 0.52 Ethyl acetate 0.73 Benzene-acetone (9:1) 0.18 Benzene-acetone (1:1) 0.88 Chloroform-methanol (19:1) 0.87 Chloroform-ethyl acetate (2:3) 0.62 Plate:
• silica gel plate (kieselgel 60, Merck, Germany) ______________________________________
• 7. Color reactions:
• ______________________________________ Color reagent ______________________________________ Sulfuric acid Positive (brown) Aniline-phthalic acid Positive (indigo blue) Vanillin-sulfuric acid Positive (violet) Molisch Negative Dragendorff Positive (light orange) Barton Negative Molybdenic acid Negative Benzidine Negative Ninhydrine Negative Alkaline potassium Negative permanganate ______________________________________
• 8. Solubilities:
• Soluble in methanol, ethanol, acetone, ethyl acetate, ethyl ether, chloroform, benzene and carbon tetrachloride;
• sparingly soluble in cyclohexane;
• and very hardly soluble in water and petroleum ether;
• and a pharmaceutical acceptable salt thereof.