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US 4,069,106 AGranted
Immobilization of enzymes on keratin
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsWilliam L. Stanley, Glenn G. Watters, Bock G. Chan
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Abstract
Insolubilized but active enzymes are prepared by mixing an aqueous solution of the enzyme with reduced keratin-containing material. The invention is particularly useful for insolubilizing sulfhydryl-containing enzymes such as urease.
Claims1
A process for preparing an insoluble but active enzyme, which comprises --
- a. reacting keratin-containing material with a sulfur-containing, reductive, disulphide-splitting agent to reduce keratin and produce a reduced keratin-containing material,
- b. dissolving a soluble sulfhydryl group containing enzyme in water, and
- c. mixing said reduced keratin-containing material with the solution of the enzyme in water so that the enzyme reacts directly with the reduced keratin and becomes chemically bonded to said reduced keratin-containing material.
Dependencies are inferred from the claim text.