Antihemophilic agent and process for its manufacture
AssigneeBehringwerke Aktiengesellschaft
InventorsHorst Schwinn, Norbert Heimburger
Life of this patent
Estimating the term…
Abstract
The invention relates to an antihemophilic agent, which may contain factor VIII, but also factor IV, VII and X activities and to a process for its manufacture by extracting placentae with an aqueous hypotonic medium, increasing the density of the extract and obtaining the supernatant layer forming thereupon.
Claims16
A method for making an antihemophilic agent, which method comprises comminuting placental tissue, washing the comminuted tissue with an aqueous hypotonic medium at a slightly acid to alkaline pH until the tissue is free of blood, extracting the washed tissue at a pH above 4.5 with an aqueous solution having an osmolarity below 330 nanomoles per liter, removing residual tissue from the extract, adding an inert water-soluble compound to the extract, whereby its density is increased, until separation and the flotation of a supernatant layer occur, and then isolating the supernatant layer, which contains said antihemophilic agent.
Dependencies are inferred from the claim text.