Indirect solid surface test for antigens or antibodies
AssigneeInternational Diagnostic Technology, Inc.
InventorGunner Bolz
Life of this patent
Estimating the term…
Abstract
An indirect method for the quantitation of an antigen or antibody in a liquid sample. In the antigen assay, antigen of the same type as the sample antigen is adsorbed onto a solid support surface. Then, specific labelled antibody is immunologically reacted in solution with the sample antigen and the adsorbed antigen. The quantity of reacted labelled antibody on the solid support surface is then determined, as for example, fluorogen label by a fluorometer. The procedure is employed for detection of antibody by reversing the antigen and antibody components in each instance.
Claims28
In an indirect method for the quantitation of antigen in a liquid sample, the steps of
- a. sorbing antigen of the same immunological type as said sample antigen onto a sorptive solid support surface,
- b. immunologically reacting a known quantity of specific labelled antibody in solution with said sample antigen in said solution and with said sorbed antigen, said labelled antibody being in excess of said sample antigen, so that a portion of said labelled antibody is bound in said solution to said sample antigen and excess labelled antibody immunologically reacts with the sorbed antigen on said surface,
- c. washing said surface, and
- d. measuring the quantity of reacted labelled antibody on said surface.
In an indirect method for the quantitation of antibody in a liquid sample, the steps of
- a. sorbing antibody of the same immunological type as said sample antibody to a sorptive solid support surface,
- b. immunologically reacting a known quantity of specific labelled antigen in solution with said sample antibody and with said sorbed antibody, said labelled antigen being in excess of said sample antibody so that a portion of said labelled antigen is bound to said sample antibody in solution and excess labelled antigen immunologically reacts with the sorbed antibody on said surface,
- c. washing said surface, and
- d. measuring the quantity of reacted labelled antigen on said surface.
An immunological system for the quantitation of antigen in a liquid sample comprising:
- a. a contained liquid volume including one quantity of labelled antibody immunlogically bound to sample antigen dispersed in said liquid volume, and
- b. antigen of the same immunological type as said sample antigen sobed directly to a solid support surface disposed in said liquid volume, said sorbed antigen being directly immunologically bound to another quantity of said labelled antibody.
An immunological system for the quantitation of antibody in a liquid sample comprising:
- a. a contained liquid volume including one quantity of labelled antigen immunologically bound to sample antibody dispersed in said liquid volume, and
- b. antibody of the same immunological type as said sample antibody sorbed directly to a solid support surface disposed in said liquid volume, said sorbed antibody being directly immunologically bound to another quantity of said labelled antigen.
Dependencies are inferred from the claim text.