Process and reagent for determining triglycerides
AssigneeBoehringer Mannheim GmbH
InventorsElli Rauscher, Erich Bernt, Wolfgang Gruber, Helmut Determann
Life of this patent
Estimating the term…
Abstract
Triglycerides are determined by enzymatic saponification with lipase in a buffer in the presence of a carboxylesterase and an alkali metal or alkaline earth metal (10-15 carbon atom) alkyl sulfate, in the additional presence of a compound of the general formula RO(CH.sub.2 CH.sub.2).sub.n H, in which R is alkyl or alkenyl containing 14 to 20 carbon atoms and n is a number of from 7 to 20; this determination prevents turbidity and does not disadvantageously influence enzyme activity.
Claims16
Process for the determination of triglycerides comprising enzymatically saponifying the triglycerides with lipase in the buffer in the presence of a carboxylesterase and of an alkali metal of alkaline earth metal alkyl sulfate, the alkyl radical of which contains 10 to 15 carbon atoms, in the additional presence of a compound of the formula RO(CH.sub.2 CH.sub.2 O).sub.n H, in which R is alkyl or alkenyl of from 14 to 20 carbon atoms and n is an integer of from 7 to 20.
Reagent for the determination of triglycerides, comprising lipase, carboxylesterase, an alkali metal or alkaline earth metal alkyl sulfate of from 10 to 15 carbon atoms in the alkyl radical, a buffer, a system for the detection of glycerol, and a compound of the general formula RO(CH.sub.2 CH.sub.2 O).sub.n H wherein R is alkyl or alkenyl of from 14 to 20 carbon atoms and n is an integer of from 7 to 20.
Dependencies are inferred from the claim text.