Loading patent…
OwnerAlexandr Pavlovich Bashkovich, Evgenia Semenovna Zimnukhova +6inventors
InventorsAlexandr Pavlovich Bashkovich, Evgenia Semenovna Zimnukhova, Agnessa Vladimirovna Prokopovich, Olga Grigorievna Polatovshaya, Maria Ivanovna Pronina, Valter Osvaldovich Kulbakh, Khasya Ionovna Rivkina-Pevtsova, Grigory Efimovich Grinberg
Estimating the term…
An enzyme of hydrolytic action, according to the invention, is a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at 40.degree. - 45.degree. C, the stability range in aqueous solutions at pH 6.8 - 8.0, quickly inactivating in said solutions at temperatures above 50.degree. C. The enzymatic preparation containing the enzyme of hydrolytic action in an amount of 50 - 500 PU/g and the metabolites of the strains Streptomyces hygroscopicus. A method for preparing the hydrolytic enzyme, in which the strain Streptomyces hygroscopicus is cultivated on media containing assimilable sources of carbon and nitrogen, inorganic mineral salts, in deep aerobic conditions, with accumulation of the enzyme of hydrolytic action and the metabolites of the said strains in the culture fluid, followed by subsequent isolation of the enzyme. A medicinal preparation for treating respiratory diseases, purulent wounds and thermal burns, for treating and preventing empyema of the pleura, containing the enzyme of hydrolytic action in the quantity of 1 - 50 PU/mg, inactive protein and mineral salts.
An enzyme of hydrolytic action which is produced by cultivation of Streptomyces hygroscopicus, Strain No. 1913 (All-Union Research Institute of Antibiotics, Moscow, Collection of Cultures, Deposit No. 997) or its variant No. 149 (USSR Research Technological Institute of Antibiotics and Enzymes for Medical Use, Leningrad, Collection of Cultures, Deposit No. 0879), said enzyme being a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at a temperature of 40.degree.-45.degree. C, the stability range in aqueous solutions at pH from 6.8 to 8.0, and quickly inactivating in said solutions at a temperature above 50.degree. C.
An enzymatic preparation which consists of the enzyme of hydrolytic action produced by cultivation of Streptomyces hygroscopicus, Strain No. 1913 (All-Union research Institute of Antibiotics, Mowcow, Collection of Cultures, Deposit No. 997) or its variant No. 149, (USSR Research Technological Institute of Antibiotics and Enzymes for Medical Use, Leningrad, Collection of Cultures, Deposit No. 0879), said enzyme being a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at temperatures 40.degree.-45.degree. C, the stability range in aqueous solutions at pH from 6.8 to 8.0, and quickly inactivating in said solutions at temperatures above 50.degree. C, taken in amount of 50-500 PU/g, and the metabolites of the strain Streptomyces hygroscopicus producing the enzyme.
A method for preparing the enzyme of hydrolytic action which is a neutral protease having the molecular weight of about 37,000, the isoelectric point at pH 8.8, the optimum activity at temperatures from 40.degree. to 45.degree. C, the stability range in aqueous solutions at pH from 6.8 to 8.0, quickly inactivating in said solutions at temperatures above 50.degree. C, in which Streptomyces hygroscopicus, Strain No. 1913 (All-Union Research Institute of Antibiotics, Moscow, Collection of Cultures, Deposit No. 997) or its variant No. 149 (USSR Research Technological Institute of Antibiotics and Enzymes for Medical Use, Leningrad, Collection of Cultures, Deposit No. 0879) is cultivated in media containing assimilable sources of carbon and nitrogen, mineral salts, in deep aerobic conditions, with accumulation in the culture fluid of the enzyme of hydrolytic action and the metabolites of the said strains with subsequent isolation of the enzyme.
Dependencies are inferred from the claim text.