Lipolytic enzyme flavoring system
AssigneeGB Fermentation Industries, Inc.
InventorsLouis I. Feldman, J. Gordon Dooley
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Abstract
A lipolytic enzyme preparation having 1 part of lipase activity to from 1 to about 10 parts of esterase activity is obtained from Mucor miehei. The enzyme preparation can be recovered substantially free of Mucor miehei rennet, and is useful for treating various triglyceride fats to provide desirable flavors.
Claims21
A method for producing a lipolytic enzyme system having esterase and lipase activity in a ratio of from 1 part of lipase activity to from 1 to about 10 parts of said esterase activity, said method comprising culturing Mucor miehei on a nutrient medium and recovering said lipolytic enzyme system substantially free of Mucor miehei rennet activity.
In the method for producing lipolyzed triglyceride fat-containing foods of enhanced flavor which comprises developing the flavor of said food by contacting said food with a lypolyzing amount of an animal-derived lipolytic enzyme agent, the improvement comprising using in place of at least a portion of said enzyme agent a lipolytic enzyme system having esterase and lipase activity in a ratio of from one part of said lipase activity to between 1 to about 10 parts of said esterase activity, said system obtained by culturing Mucor miehei on a nutrient medium and recovering said system substantially free of Mucor miehei rennet activity.
A method for producing a lipolytic enzyme system having both esterase activity and lipase activity in a ratio of about from 2 to 10 parts of esterase activity to one part of lipase activity, said method comprising (a) culturing Mucor miehei on a nutrient medium to produce a mash;
- (b) filtering the mash at a acid pH, thus producing a filter cake;
- (c) extracting the filter cake at alkaline pH;
- and (d) acidifying the extract to a pH of about 7, thus obtaining a lipolytic enzyme substantially free of rennin and having both esterase activity and lipase activity in a ratio of about from 1 to 10 parts of esterase activity to one part of lipase activity.
Dependencies are inferred from the claim text.