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AssigneeBehringwerke Aktiengesellschaft
InventorsHans Bohn, Ferdinand Stutzinger
Estimating the term…
A pregnancy-specific .beta..sub.1 -glycoprotein, characterized by An electrophoretic migration speed in agar gel in the range of the .beta..sub.1 -globulins, A sedimentation constant of 4.6 S .+-. 0.5 S, a molecular weight of 100 000 .+-. 15 000, An extinction coefficient E.sub.1cm.sup.1% of 11.6 .+-. 0.5 (278 m.mu.; 1/15-molar phosphate buffer, pH 7.0), a carbohydrate content of 28.05 .+-. 1.55%, comprising 10.7 .+-. 1.0% of hexoses, 10.0 .+-. 0.5% of hexosamine, 0.55 .+-. 0.05% of fucose and 7.0 .+-. 0.5% of neuraminic acid, And process for isolating it from the placenta or blood or urine of pregnant women. This protein may be used as a reagent and for the preparation of antisera as reagents for the detection of pregnancy and for pre-natal supervision.
A purified, isolated pregnancy-specific .beta..sub.1 -glycoprotein characterized by an electrophoretic migration speed in agar gel in the range of the .beta..sub.1 -globulins,
A process for isolating a pregnancy-specific .beta..sub.1 -glycoprotein, which comprises
A process for isolating a pregnancy-specific .beta..sub.1 -glycoprotein which comprises contacting the blood serum, urine or an extract, in a weak salt solution. of the comminuted placentas of pregnant women with an antibody of said pregnancy specific .beta..sub.1 -glycoprotein bound to a solid carrier at a pH between 4 and 9 and subsequently eluting the glycoprotein from the carrier at a pH between 2 and 4 or by treatment with a protein dissociating compound.
Process for the purification of an isolated pregnancy-specific .beta..sub.1 -glycoprotein, wherein said glycoprotein in an aqueous solution containing phosphate is contacted with hydroxylapatite and subsequently the hydroxylapatite is separated from the aqueous medium containing the glycoprotein.
Dependencies are inferred from the claim text.