Graft thin layer chromatography
AssigneeUniversity of Illinois Foundation
InventorsRamesh C. Pandey, Kenneth L. Rinehart, Jr.
Life of this patent
Estimating the term…
Abstract
A novel plate system for thin layer chromatography comprises a supporting plate coated over about 50-90% of its area with an adsorbent layer, the uncoated surface comprising at least one strip of uniform width along an end of the supporting plate. Two such plates grafted and clamped in a lap-joint so that the edges of the adsorbent layers are in intimate contact provide an assembly on which a chromatogram developed on the first plate can be transferred in whole or in part onto the second plate. The assembly and technique are particularly useful for preparative thin layer chromatography.
Claims16
A TLC plate system for graft thin-layer chromatography comprising a rectangular supporting plate and an adsorbent layer thereon coating about 50% to about 90% of the surface of the supporting plate, the uncoated surface comprising at least one strip of uniform width along an end of the supporting plate and the adsorbent edge adjacent to the uncoated surface being true and square to assure full and intimate contact between adsorbent layers when joined to another of said plate systems in a lap joint.
A TLC plate system for graft thin-layer chromatography comprising:
- a. a pair of lap-joined plates each of which plates comprises a rectangular supporting surface and an adsorbent layer thereon coating about 50% to about 90% of the surface of the supporting plate, the uncoated surface comprising at least one strip of uniform width along an end of the supporting plate;
- and
- b. clamping means for securing the plates at the lap-joint formed when the uncoated strip of the first plate is placed over the adsorbent layer of the second plate in a manner such that the adsorbing layers of the two adjoining plates are in intimate contact along their entire length so as to permit a chromatogram developed initially on the first plate to be transferred, in part or in whole, onto the adsorbent layer of the second plate.
A method of transferring a chromatogram of a mixture of compounds from a first plate to a second plate each of said plates comprising a rectangular supporting plate and an adsorbent layer thereon coating about 50% to about 90% of the surface of the supporting plate, the uncoated surface comprising at least one strip of uniform width along an end of the supporting plate, which method comprises:
- a. joining and securing the two plates in a lap-joint which is formed when the uncoated strip of the first plate is placed over the adsorbent layer of the second plate in a manner such that the adsorbing layers of the two joining plates are in intimate contact along their entire length;
- b. applying a solution of the mixture of compounds to be chromatogrammed to the adsorbent layer of the first plate;
- and
- c. developing the chromatogram for a time sufficient to transfer at least a portion of the chromatogram from the first plate to the second plate.
Dependencies are inferred from the claim text.