Production of antibiotic FR-02A (efrotomycin) by streptomyces lactamdurans
AssigneeMerck & Co., Inc.
InventorsWilliam M. Maiese, Richard G. Wax
Life of this patent
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Abstract
An antibiotic, designated FR-02A or efrotomycin, is obtained by culturing Streptomyces lactamdurans in a fermentation broth and extracting the whole broth with a water immiscible polar organic solvent to obtain the antibiotic FR-02A.
Claims11
A method of producing antibiotic FR-02A which comprises cultivating the microorganism Streptomyces lactamdurans NRRL 3802 in a fermentation broth containing a nutrient sodium composed of assimilable sources of carbohydrate, nitrogen, and inorganic salts wherein the nutrient medium contains between about 1% and 6% by weight of carbohydrate and between about 0.2% and 6% by weight of available nitrogen under aerobic conditions at a temperature in the range of from about 26.degree. C. to 30.degree. C. for a period of from about two to five days wherein in the pH of the aqueous nutrient medium is in the range of from about 6.0 to 8.0 and extracting the whole broth with a water immiscible polar organic solvent selected from the group consisting of methyl formate, ethyl formate, methyl acetate, ethyl acetate, n-butyl acetate, isobutyl acetate, ethyl propionate, cyclohexanone, chloroform, methylene chloride, carbon tetrachloride, ethylene dichloride, 1-chloro-2,2-dimethylpropane, tetrachloroethylene, and bromoform to obtain the antibiotic FR-02A.
A method of producing antibiotic FR-02A which comprises cultivating the microorganism Streptomyces lactamdurans NRRL 3802 in a fermentation broth containing a nutrient medium which contains suspended nutrient matter, said nutrient medium comprised of assimilable sources of carbohydrate, nitrogen, and inorganic salts wherein the nutrient medium contains between about 1% and 6% by weight of carbohydrate and between about 0.2% and 6% by weight of available nitrogen under aerobic conditions at a temperature in the range of from about 26.degree. C. to 30.degree. C. for a period of from about two to five days wherein the pH of the aqueous nutrient medium is in the range of from about 6.0 to 8.0, separating the solids from the broth and extracting the solids with a polar organic solvent selected from the group consisting of methyl formate, ethyl formate, methyl acetate, ethyl acetate, n-butyl acetate, isobutyl acetate, ethyl propionate, acetone, methyl ethyl ketone, cyclohexanone, chloroform, methylene chloride, carbon tetrachloride, ethylene dichloride, 1-chloro-2,2-dimethylpropane, tetrachloroethylene, and bromoform to obtain the antibiotic FR-02A.
Dependencies are inferred from the claim text.