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US 4,065,310 AGranted
Fixing and retrieving recorded information produced by means of photosensitive material of biological origin
OwnerEsther Dujardin, Ysbrand Kuiper +2inventors
InventorsEsther Dujardin, Ysbrand Kuiper, Rene Cremer, Cyrille Sironval
Life of this patent
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Abstract
A method of recording and reproducing information using a photographic recording material which comprises a support bearing at least one photosensitive layer containing photoactive protochlorophyll(ide)-apoprotein complex. The layer is exposed to light of the wavelength region absorbed by the complex in a pattern representing information to be recorded and forms a corresponding pattern of differentiations in light-absorption and fluorescence-emission characteristics. The latter can be scanned to reproduce the information.
Claims8
A method of recording and reproducing information comprising the steps of:
- providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier;
- information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information;
- fixing said layer by denaturation of the protein of said photoactive product;
- and
- retrieving the recorded information from said layer by detecting a pattern of differences in intensity of light absorption of the layer;
- said photoactive product being formed by the steps of:
- extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5.degree. C;
- adding to said binary complex at a temperature of at most 5.degree. C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate;
- cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material;
- lyophilizing said product at a temperature of at most 5.degree. C so that the dried final product remains photoactive at room temperature;
- and
- providing said product in layer form suitable for recording.
9 more elements
A method of recording and reproducing information comprising the steps of:
- providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier;
- information-wise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information;
- fixing said layer by denaturation of the protein of said photoactive product;
- and
- retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer;
- said photoactive product being formed by the steps of:
- extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5.degree. C;
- adding to said binary complex at a temperature of at most 5.degree. C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate;
- cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material;
- lyophilizing said product at a temperature of at most 5.degree. C so that the dried final product remains photoactive at room temperature;
- and
- providing said product in layer form suitable for recording.
9 more elements
A method of recording and reproducing information comprising the steps of:
- providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier;
- informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information;
- fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100.degree. C;
- and
- retrieving the recorded information from said layer by detecting a pattern of differences of intensity of light absorption of the layers;
- said photoactive product being formed by the steps of:
- extracting from an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5.degree. C;
- adding to said binary complex at a temperature of at most 5.degree. C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate;
- cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material;
- lyophilizing said product at a temperature of at most 5.degree. C so that the dried final product remains photoactive at room temperature;
- and
- providing said product in layer form suitable for recording.
9 more elements
A method of recording and reproducing information comprising the steps of:
- providing a photosensitive layer of protochlorophyll(ide)-apoprotein photoactive product upon a layer carrier;
- informationwise exposing said layer to light of a wavelength absorbed by said complex thereby forming a pattern of differentials in light-absorption and fluorescence emission characteristics representing the exposition pattern to record information;
- fixing said layer by subjecting said layer subsequent to recordal of information thereon to a temperature below -100.degree. C;
- and
- retrieving the recorded information from said layer by subjecting the layer to light and measuring fluorescence emission from said layer;
- said photoactive product being formed by the steps of:
- extraction of an etiolated plant material a photoactive protochlorophyll(ide) apoprotein binary complex with a buffer solution at a pH between 7 and 10 in the presence of an agent to protect the apoprotein against denaturation at a temperature of at most 5.degree. C;
- adding to said binary complex at a temperature of at most 5.degree. C a natural or synthetic polymeric material selected from the group consisting of polyethylene glycol, polyvinylpyrrolidone, triethanolamine, saccharose and dextran to form a precipitate;
- cold-centrifuging said precipitate to form a product which is either in complex or in association with said polymeric material;
- lyophilizing said product at a temperature of at most 5.degree. C so that the dried final product remains photoactive at room tepmerature;
- and
- providing said product in layer form suitable for recording.
9 more elements
Dependencies are inferred from the claim text.