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US 4,064,011 AGranted
Process for producing ECoRI restriction endonuclease with E.coli mutants
AssigneeGesellschaft fur Biotechnologische Forschung mbH
InventorsHubert Mayer, Horst Schutte
Life of this patent
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Abstract
ECoRI restriction endonuclease is produced with certain mutant strains of E.coli which are not antibiotic resistant and do produce the said enzyme which is obtained from the E.coli mutant cells without destroying the cells by extraction procedures.
Claims8
A process of producing ECoRI restriction endonuclease which comprises cultivating E.coli SB5, DSM No. 686 in an aqueous nutrient medium therefor and recovering said enzyme from the cultivated cells.
Dependencies are inferred from the claim text.