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US 4,063,017 AGranted
Porous cellulose beads and the immobilization of enzymes therewith
AssigneePurdue Research Foundation
InventorsGeorge T. Tsao, Li Fu Chen
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Abstract
Porous cellulose beads are prepared by distributing droplets of a solvent mixture containing a cellulose derivative into an aqueous solution to form porous beads which are then washed and hydrolyzed to form porous cellulose beads. The porous cellulose beads, which may be cross-linked, if desired, by suitable treatment, are useful carriers to which enzymes can be immobilized.
Claims22
A process for the preparation of porous cellulose beads suitable for use as a carrier of enzymes and other biological agents which comprises the steps of:
- a. dissolving a hydrolyzable cellulose derivative in an inert organic, water-miscible solvent selected from the group of a mixture of acetone and dimethylsulfoxide and a mixture of acetone and formamide to form a solution having a density greater than that of a precipitation solution the cellulose derivative to solvent ratio ranging from 1:20 to 1:3 weight/volume;
- b. distributing said solution in the form of droplets into a precipitation solution whereby said cellulose derivative is precipitated in the form of uniformly porous beads;
- c. separating the precipitated beads from said solution;
- d. washing the separated porous beads with water;
- e. hydrolyzing the washed beads to convert the beads to cellulose and to increase the active sites for attachment of enzymes and other biological agents;
- f. washing the hydrolyzed beads to obtain porous cellulose beads having a uniformly distributed void space greater than 50% by volume.
3 more elements
Dependencies are inferred from the claim text.