Immunoassay involving the binding of RF to the antigen-antibody complex
AssigneeTechnicon Instruments Corporation
InventorsPierre Lucien Masson, Joseph Felix Heremans
Life of this patent
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Abstract
A method for analyzing a biological fluid sample for Ab, Ag, or Ab:Ag complexes therein which includes the step of adding to the sample a solution of RF or Clq to bind with Ab:Ag complexes present or formed therein, and thereafter analyzing the mixture so formed for complexes bound to RF.
Claims31
A method of analyzing a biological fluid sample for Ab, Ag, or Ab:Ag complexes therein which includes the step of adding to the sample a solution of RF to bind with Ab:Ag complexes formed or present therein, and thereafter analyzing the mixture so formed for complexes bound to RF.
A method of assaying a particular Ab or Ag in a biological fluid sample, which comprises the steps of:
- a. adding to the sample an excess of the Ag or Ab which is specific to the particular Ab or Ag, respectively, in the sample to form the Ab:Ag complex;
- b. adding to the mixture formed in step (a) a known amount of a solution of RF in excess of that required to bind with all the said Ab:Ag complex present, the RF/Ab:Ag formed agglomerating in the mixture;
- c. separating from the mixture the agglomerated RF/Ab:Ag, and
- d. measuring the amount of RF remaining in the mixture after step (c) or separated from the mixture in step (c), and therefrom calculating the amount of particular Ab or Ag present in the original sample.
A method of assaying a biological fluid sample to detect the presence of Ab:Ag complexes therein, which comprises the steps of:
- a. adding to the sample a known amount of a solution of RF in excess of the amount required to bind with all the Ab:Ag complexes in the sample to form RF/Ab:Ag, the said RF/Ab:Ag agglomerating in the mixture;
- b. separating from the mixture the agglomerated RF/Ab:Ag;
- and
- c. measuring the amount of RF remaining in the mixture after step (b) or separated from the mixture in step (b), and therefrom calculating the amount of Ab:Ag complexes present in the original sample.
A method of assaying a particular Ab or Ag in a biological fluid sample, which comprises the steps of:
- a. adding to the sample an excess of an Ag' or Ab' which is specific to particular Ab or Ag, respectively, in the sample, to form an Ab:Ag' complex or Ab':Ag complex, the Ab' or Ag' carrying an identifying label;
- b. adding to the mixture formed in step (a) a solution of RF in an amount at least sufficient to bind with all the Ab:Ag' or Ab':Ag complex in the mixture;
- and
- c. measuring the amount of Ab' or Ag' free in the mixture or bound to the RF, and therefrom calculating the amount of the particular Ab or Ag present in the original sample.
A method of assaying a biological sample to detect the presence of Ab:Ag complexes therein, which comprises the steps of:
- a. adding to the sample a known amount of an Ab':Ag complex which carries an identifying label, and a solution of RF in an amount insufficient to bind with the total amount of Ab:Ag and Ab':Ag' complexes in the mixture formed, and
- b. measuring the amount of Ab':Ag' free in the mixture or bound to the RF and therefrom calculating the amount of Ab:Ag complexes present in the original sample.
A method of assaying a particular Ab or Ag in a biological fluid sample, which comprises
- a. adding to the sample an Ag or Ab which is specific to the particular Ab or Ag, respectively, in the sample to form an Ab:Ag complex;
- b. adding to the mixture from step (a) a known amount of the particular Ab or Ag to be determined, which amount carries an identifying label;
- c. adding to the mixture formed in step (b) a solution of RF in an amount at least sufficient to bind with all the Ab:Ag complex in the mixture;
- and
- d. measuring the amount of labelled Ab or Ag remaining free in the mixture or bound to the RF.
A method of detecting the presence in, or absence from, a biological fluid sample of Ab:Ag complexes, which comprises adding to the sample a solution of RF and a material which is caused to agglutinate on contact with RF, and detecting the degree to which agglutination of the material occurs compared with a standard mixture of said material and RF.
A method of immunoassay of a biological fluid sample for Ab, Ag or Ab:Ag complexes, which includes the steps of
- a. adding to the sample an Ab, Ag or Ab:Ag complex carrying an enzyme label;
- b. adding a solution of RF, to bind with any Ab:Ag complex present;
- and
- c. using the inhibitory effect of RF on the enzymic activity of any labelled Ab:Ag complex bound thereto, in order to assay the sample.
A method of continuous flow analysis of biological fluid samples which includes the step of adding to the samples a solution of RF to bind with Ab:Ag complexes in or formed in the samples, and thereafter treating the mixture so formed to detect the Ab:Ag complexes bound to RF.
A method of detecting the presence in or absence from a biological fluid sample of Ab:Ag complexes, comprising adding to the sample a known amount of RF and a known amount of latex particles coated with IgG whereby RF becomes bound to any Ab:Ag complex present and the remainder of the RF causes agglutination of the latex particles;
- and determining the amount of said remainder of RF by counting the agglutinated latex particles and comparing the result with a standard curve.
Dependencies are inferred from the claim text.