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US 4,062,731 AGranted
Production of uricase from micrococcus luteus
AssigneeEastman Kodak Company
InventorsRoy Eugene Snoke, Hugh Arthur Risley, Charles Thomas Goodhue
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Abstract
A stable uricase preparation of high activity is produced by growing Micrococcus luteus NRRL B-8166 in a nutrient medium. Uricase preparations having activities of up to about 1000 U/liter are produced.
Claims8
A process for producing uricase which comprises growing Micrococcus luteus NRRL B-8166 in a medium to produce to uricase, and separating the uricase from the medium.
A uricase preparation comprising an uricase having the following properties:
- a molecular weight of about 97,000 daltons;
- maximum activity at a pH of 8.6, in a potassium phosphate buffer;
- a Michaelis constant of 3.7 .times. 10.sup.-5 ;
- an inhibition by Fe.sup.+3, Cn.sup.+2, Co.sup.+2, or Mn.sup.+2 ;
- a biphasic inhibitory response rate curve to Co.sup.+2 or Mn.sup.+2 ;
- and
- activation by PO.sub.4.sup.-3 or SO.sub.4.sup.-2.
4 more elements
An uricase preparation comprising a uricase
- a molecular weight of about 97,000 daltons;
- a maximum activity at a pH of 8.6, in a potassium phosphate buffer;
- a Michaelis constant of 3.7 .times. 10.sup.-5 ;
- an inhibition by Fe.sup.+3, Cn.sup.+2, or Mn.sup.+2 ;
- a biphasic inhibitory response rate curve to Co.sup.+2 or Mn.sup.+2 ;
- and
- activiation by PO.sub.4.sup.-3 or SO.sub.4.sup.+2 and obtained by growing Micrococcus luteus NRRL B-8166.
4 more elements
Dependencies are inferred from the claim text.