Selective adsorbent for use in affinity chromatography
AssigneePierce Chemical Company
InventorsRoy Oliver, Garfield P. Royer
Life of this patent
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Abstract
A new selective adsorbent for use in affinity chromatography. This adsorbent comprises, as one part, a water soluble covalently bonded complex of an affinity ligand and a biospecific handle. The other part thereof is an insoluble matrix or solid support which has covalently immobilized on its surface an adsorbent which has specific affinity for the biospecific handle of the complex. Attachment between the complex, containing the ligand, and the support is achieved by reversible coupling between the adsorbent and the biospecific handle and, as a result thereof, the ligand is not covalently bonded to the support. In turn, substantially complete recovery of the enzyme from the column can be effected.
Claims5
A selective adsorbent for use in affinity chromatography comprising, as one part, a water-soluble covalently bonded complex of an affinity ligand and a biospecific handle and, as the other part, an insoluble matrix or solid support which has covalently immobilized on its surface an adsorbent which has specific affinity for the biospecific handle of the complex, the biospecific handle and the absorbent being polynucleotides or 2' deoxy derivatives thereof, the polynucleotide of said handle having a base which is complementary, as to spatial arrangement and affinitive interaction, with the base of the polynucleotide adsorbent.
Dependencies are inferred from the claim text.