Apparatus for deflection electrophoresis
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsKurt Hannig, Hanns Wirth
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Abstract
In a procedure for performing a continuous, free-film deflection electropesis in which a narrow stream of a sample mixture is introduced into a thin strip of a buffer solution flowing through a separating chamber while an electric field is applied transversely to the flow direction, band broadening of the sample stream fractions is reduced by providing suitably selected values for the temperature gradient in the chamber and the wall zeta. Additional improvements are achieved by constructing the buffer solution flow path in the region of sample introduction in order to increase the buffer solution velocity thereat.
Claims12
In apparatus for performing a continuous, free-film deflection electrophoresis procedure, the apparatus including:
- means defining a separating chamber having two spaced parallel walls delimiting opposite boundaries of a separating chamber gap, and two electrode chambers each disposed adjacent a respective opposite, lateral edge of the gap;
- two ion transmitting membranes each separating a respective electrode chamber from the separating chamber gap;
- first supply means for supplying a buffer solution to one end of the gap so that the solution flows in the form of a thin layer through the gap;
- second supply means disposed downstream of the first supply means for introducing into the thin buffer solution layer a spatially narrowly defined stream of a sample mixture;
- voltage applying means arranged to produce an electric separating field extending transversely to the direction of buffer solution flow and parallel to the two spaced walls for causing the sample stream to be divided into fractions as it traverses the length of the gap, the voltage applying means being composed of two electrodes each disposed in a respective one of the electrode chambers;
- and means for analyzing such fractions, the improvement wherein said apparatus comprises third supply means for introducing controlled quantities of an adjuvant fluid into the buffer solution upstream of the point of introduction of the sample mixture into the buffer solution layer, and wherein said means for analyzing comprises:
- a light source disposed to one side of said separating chamber gap;
- and a series of photodiodes disposed to the other side of said separating chamber gap and extending transversely to the direction of buffer solution flow through said gap;
- a shift register having a plurality of stages each operatively coupled to a respective one of said photodiodes in a manner to permit each said stage to store signals corresponding to the radiation energy impinging on the associated photodiode, and an interrogation device operatively coupled to said shift register for serially reading out such stored signals.
In apparatus for performing a continuous, free-film deflection electrophoresis procedure, the apparatus including:
- means defining a separating chamber having two spaced parallel walls delimiting opposite boundaries of a separating chamber gap, and two electrode chambers each disposed adjacent a respective opposite, lateral edge of the gap;
- two ion transmitting membranes each separating a respective electrode chamber from the separating chamber gap;
- first supply means for supplying a buffer solution to one end of the gap so that the solution flows in the form of a thin layer through the gap;
- second supply means disposed downstream of the first supply means for introducing into the thin buffer solution layer a spatially narrowly defined stream of a sample mixture;
- voltage applying means arranged to produce an electric separating field extending transversely to the direction of buffer solution flow and parallel to the two spaced walls for causing the sample mixture stream to be divided into fractions as it traverses the length of the gap, the voltage applying means being composed of two electrodes each disposed in a respective one of the electrode chambers;
- and means for analyzing such fractions, the improvement wherein said means for analyzing comprises:
- a light source disposed to one side of said separating chamber gap;
- and a series of photodiodes disposed to the other side of said separating chamber gap and extending transversely to the direction of buffer solution flow through said gap;
- a shift register having a plurality of stages each operatively coupled to a respective one of said photodiodes in a manner to permit each said stage to store signals corresponding to the radiation energy impinging on the associated photodiode, and an interrogation device operatively coupled to said shift register for serially reading out such stored signals.
Dependencies are inferred from the claim text.