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US 4,061,543 AGranted
Bioassay for drugs and antibiotics
AssigneeGeneral Electric Company
InventorsCharles P. Bean, Roy J. King, Jr., Egidijus E. Uzgiris
Life of this patent
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Abstract
Effectiveness on cellular processes of a drug or antibiotic introduced into isotonic sucrose solution of 0.005 Normal sodium chloride containing physiological cells in suspension is determined by irradiating the solution, situated in an electric field, with a laser beam and measuring frequency difference between incident light and light scattered by cell motion in the electric field.
Claims6
A method of performing a bioassay to test for effectiveness of medicinal substances upon cell mobility comprising:
- suspending biological cells to be monitored in isotonic sucrose solution substantially in the range of .02 to .001 Normal sodium chloride;
- establishing an electric field across at least a portion of said solution;
- introducing into said solution over a finite period of time a medicinal substance to be examined for efficacy;
- irradiating said solution within said electric field with a beam of coherent light;
- detecting light scattered by at least some of said cells suspended in said solution;
- varying amplitude of said electric field as said medicinal substance to be evaluated encounters said cells suspended in said solution so as to maintain at maximum a signal corresponding to a predetermined frequency difference between the incident beam of coherent light and said light scattered by at least some of said cells suspended in said solution;
- and
- measuring the resulting changes in electric field amplitude required to maintain said signal at maximum.
5 more elements
Dependencies are inferred from the claim text.