Cholesterol oxidaze and process for preparing same
AssigneeKikkoman Shoyu Co., Ltd.
InventorsFumihiko Yoshida, Kiyoshi Mizusawa, Kazuo Nakamura
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Abstract
A novel cholesterol oxidase can be obtained by culturing a strain belonging to the Genus Corynebacterium and having an ability to produce cholesterol oxidase in a medium containing cholesterol.
Claims10
Cholesterol oxidase which is produced by a microorganism belonging to the species Corynebacterium cholesterolicum, has a molecular weight of 57,000 as measured by Weber's SDS disk electrophoresis, and oxidizes the hydroxyl group attached to the 3.beta.-position of steroids and exerts no action upon the hydroxyl groups attached to other positions nor upon esters.
A process for preparing cholesterol oxidase which comprises culturing, in a medium in the presence of cholesterol, a microorganism belonging to the species Corynebacterium cholesterolicum and having an ability to produce cholesterol oxidase and collecting the cholesterol oxidase from the cultured medium.
Dependencies are inferred from the claim text.