Tissue culture technique utilizing a specific light source
AssigneeGTE Laboratories Incorporated
InventorPrakash G. Kadkade
Life of this patent
Estimating the term…
Abstract
One aspect of the invention comprises a method for stimulating organogenesis of explants in a tissue culture comprising the step of illuminating the explant culture during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm. A second aspect of the invention includes the additional illumination of the explants with 740nm light, subsequent to an intervening period of preferably one to two weeks. The 660nm light primarily stimulates shoot formation while the 740nm light primarily stimulates root formation. The intervening period precludes photoreversibility of the initially obtained light effects.
Claims11
A method for stimulating organogenesis of explants in a tissue culture medium comprising the step of:
- illuminating the explant during at least a portion of its differentiation stage with light having a predominate spectral emission at approximately 660nm.
A method for stimulating organogenesis of explants in a tissue culture medium comprising the steps of:
- placing an embryo of the donor plant in a culture medium;
- illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 660nm to induce shoot formation;
- and
- illuminating the cultured embryo with light having a predominate spectral emission at a wavelength of approximately 740nm to stimulate root formation, an intervening period being provided between the two illuminations sufficiently long to prevent photoreversibility of the firstly stimulated effect.
A method for stimulating organogenesis of embryo explants in a tissue culture medium comprising the steps of:
- placing only the distal or cotyledon end of the embryo in the culture medium and
- illuminating the embryo during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm.
Dependencies are inferred from the claim text.