Serological reagent and preparation thereof
AssigneeTakeda Chemical Industries, Ltd.
InventorsYukio Sato, Seijun Wada, Shigetaka Matsuzawa
Life of this patent
Estimating the term…
Abstract
A latex reagent is prepared by allowing latex particles to adsorb a nonionic surfactant of ethylene oxide type, sensitizing the latex particles thus having the surfactant adsorbed with a serologically active substance such as antigen or antibody and suspending the sensitized latex particles in an aqueous solvent. The thus-prepared latex reagent has an excellent quality, e.g. both a high stability and a high sensitivity in serologic reactions, and can be employed with advantages in a broad variety of serologic reactions.
Claims14
A method for preparing a latex reagent for serologic reactions, which comprises allowing latex particles to adsorb a nonionic surfactant, sensitizing the latex particles with an antigen or antibody and suspending the sensitized latex particles in an aqueous solvent, said nonionic surfactant being selected from the group consisting of a block copolymer of ethylene oxide and polyoxypropylene glycol which contains, within it's respective molecules, about 50% to about 80% of ethylene oxide, with the molecular weight of the hydrophobic polyoxypropylene glycol units being about 950 to about 3,850;
- a polyoxyethylene alkyl ether containing about 30 moles to about 100 moles of ethylene oxide and wherein the alkyl group contains 12 to 18 carbon atoms;
- and a polyoxyethylene alkylaryl ether which contains about 30 moles to about 100 moles of ethylene oxide and wherein the alkylaryl is phenyl substituted with alkyl of 8 to 18 carbon atoms.
A latex reagent for serologic reactions, which is prepared by allowing latex particles to adsorb a nonionic surfactant, sensitizing the latex particles with an antigen or antibody and suspending the sensitized latex particles in an aqueous solvent, said nonionic surfactant being selected from the group consisting of a block copolymer of ethylene oxide and polyoxypropylene glycol which contains, within it's respective molecules, about 50% to about 80% of ethylene oxide, with the molecular weight of the hydrophobic polyoxypropylene glycol units being about 950 to about 3,850;
- a polyoxyethylene alkyl ether containing about 30 moles to about 100 moles of ethylene oxide and wherein the alkyl group contains 12 to 18 carbon atoms;
- and a polyoxyethylene alkylaryl ether which contains about 30 moles to about 100 moles of ethylene oxide and wherein the alkylaryl is phenyl substituted with alkyl of 8 to 18 carbon atoms.
Dependencies are inferred from the claim text.