Glucose isomerization process
AssigneeR. J. Reynolds Tobacco Company
InventorMargaret E. Long
Life of this patent
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Abstract
Microbial cell material having a glucose-isomerizing enzyme associated therewith is subjected to flocculation conditions to produce a flocculated aggregate containing the cell material. Glucose syrups are brought into contact with the flocculated aggregates and a portion of the glucose is thereby converted to fructose in the presence of the glucose-isomerizing enzyme.
Claims9
A continuous process for isomerizing glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior treatment of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent followed by drying and sieving of the aggregate to produce dried aggregate particles that are approximately 10 to 30 mesh in size, said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50.degree. to 90.degree. C. and a pH of 6 to 10 to effect isomerization of a portion of the glucose and recovering an effluent syrup containing glucose and fructose.
A continuous process for converting glucose to fructose in the presence of glucose isomerase-containing microbial cells wherein said cells are used in the form of an aggregate resulting from prior flocculation of said cells with 0.5 to 50 percent by weight based on the wet weight of said cells of a polyelectrolyte flocculating agent selected from the group consisting of anionic and cationic polyelectrolytes followed by
- a. harvesting the flocculated cells,
- b. extruding the harvested flocculated cells, and
- c. drying the extruded flocculated cells to a moisture level of less than 15 percent,
- said continuous process comprising passing a glucose solution through a bed of said aggregate at a temperature of 50.degree. to 90.degree. C. and a pH of 6 to 10 to effect conversion of a portion of the glucose to fructose and recovering an effluent syrup containing glucose and fructose.
Dependencies are inferred from the claim text.