Specimen holding device and method of using same
AssigneeErnst Leitz Wetzlar GmbH
InventorsWolfgang Rapp, Karl-Heinz Haas, Heribert Luessem
Life of this patent
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Abstract
Disclosed is a specimen holding device suitable for holding fluid substances which are to be analyzed and which cause clouding or color change by reacting with a reagent, comprising a generally solid piece of transparent material containing a plurality of elongated recesses having the longitudinal axis thereof extending into the material, preferably perpendicularly to the top surface of a solid piece. Preferably, the recesses are iterconnectedat their lower ends with a filling channel which terminates on one side surface of the device. Also disclosed is a method for using the device.
Claims25
A device for holding a reagent and a fluid substance to be analyzed upon reaction with the reagent to produce a visual change, comprising:
- a. a generally horizontally axially elongated, generally solid block of a transparent material, said block having longitudinal side surfaces, at least one of said side surfaces enabling viewing through the block;
- b. plural means for holding both the reagent and the fluid substance to be analyzed, with said fluid substance overlying the upper surface of the reagent before reaction therewith, said holding means including a plurality of elongated recesses extending downwardly from the top surface of the block, said recesses being positioned to permit separate lateral viewing, transverse to said at least one side surface, of each recess and of the reaction taking place therein between the reagent and the fluid substance in a region contiguous to the surface of the reagent, and
- c. means for closing off the bottom ends of said recesses, said closing-off means including removable plug members adapted for insertion into the bottom ends of the recesses.
A device for holding a reagent and a fluid substance to be analyzed upon reaction with the reagent to produce a visual change, comprising:
- a. a generally horizontally axially elongated, generally solid block of a transparent material, said block having longitudinal side surfaces, at least one of said side surfaces enabling viewing through the block;
- b. plural means for holding both the reagent and the fluid substance to be analyzed, with said fluid, substance overlying the upper surface of the reagent before reaction therewith, said holding means including a plurality of elongated recesses extending downwardly from the top surface of the block, said recesses being positioned to permit separate lateral viewing, transverse to said at least one side surface, of each recess and of the reaction taking place therein between the reagent and the fluid substance in a region contiguous to the surface of the reagent, and
- c. means for closing off the bottom ends of said recesses, wherein said recesses comprise at least one planar side-wall surface which forms an angle different from 90.degree. with the longitudinal axis of said solid block, whereby light entering perpendicularly to said planar side-wall surface does not emanate along the line of transverse viewing.
A device for holding a reagent and a fluid substance to be analyzed upon reaction with the reagent to produce a visual change, comprising:
- a. a generally horizontally axially elongated, generally solid block of a transparent material, said block having longitudinal side surfaces, at least one of said side surfaces enabling viewing through the block;
- b. plural means for holding both the reagent and the fluid substance to be analyzed, with said fluid substance overlying the upper surface of the reagent before reaction therewith, said holding means including a plurality of elongated recesses extending downwardly from the top surface of the block, said recesses being positioned to permit separate lateral viewing, transverse to said at least one side surface, of each recess and of the reaction taking place therein between the reagent and the fluid substance in a region contiguous to the surface of the reagent, and
- c. means for closing off the bottom ends of said recesses, wherein one of the longitudinal side surfaces of said solid block is sub-divided into a plurality of segments corresponding to the number of said recesses, each segment having a wall surface arranged at a common acute angle with respect to the longitudinal axis of said solid body, whereby light entering perpendicularly to said segment wall surface does not emanate along a line of transverse viewing.
A method for analyzing a fluid substance comprising the steps of placing in each of the elongated recesses of a device comprising:
- a. a generally horizontally axially elongated, generally solid block of a transparent material, said block having longitudinal side surfaces, at least one of said surfaces enabling viewing through the block;
- b. plural means for holding both the reagent and the fluid substance to be analyzed, with said fluid substance overlying the upper surface of the reagent before reaction therewith, said holding means including a plurality of elongated recesses extending downwardly from the top surface of the block, said recesses being positioned to permit separate lateral viewing, transverse to said at least one side surface, of each recess and of the reaction taking place therein between the reagent and the fluid substance in a region contiguous to the surface of the reagent, and
- c. means for closing off the bottom ends of said recesses;
- a reagent which is reactive with said substance to produce a detectable indication function, adding said fluid substance to each recess so that the fluid substance overlies the upper surface of the reagent, whereby a detectable indication function is produced in said recesses, and observing said indication function by lateral viewing through each of said recesses.
Dependencies are inferred from the claim text.