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US 4,059,694 AGranted
Cardiotonic agent
AssigneeUniversity of Hawaii
InventorsTed R. Norton, Shoji Shibata, Midori Kashiwagi
Life of this patent
Estimating the term…
Abstract
A peptide designated anthopleurin-A, hereinafter also referred to as AP-A, obtained from the sea anemone Anthopleura xanthogrammica is found to possess cardiotonic activity.
Claims8
A peptide according to claim 1 having the amino acid sequence:
- Gly-Val-Ser-Cys-Leu-Cys-Asp-Ser-Asp-Gly-Pro-Ser-Val-Arg-Gly-Asn-Thr-Leu-Se r-Gly-Thr-Leu-Trp-Leu-Tyr-Pro-Ser-Gly-Cys-Pro-Ser-Gly-Trp-His-Asn-Cys-Lys-A la-His-Gly-Pro-Thr-Ile-Gly-Trp-Cy-Cys-Lys-Gln.
A process for the preparation of anthopleurin-A from sea anemone, wherein the concentration of the intial alcohol extract is carried out at below 40.degree. C and all other steps are carried at room temperature or below which comprises:
- a. extracting the sea anemones with water or with an aqueous-alcoholic mixture;
- b. subjecting the extract to gel filtration chromatography on a column of cross-linked dextran eluted with NH.sub.4 HCO.sub.3 solution and collecting and lylophilizing the active fraction;
- c. subjecting the active fraction to chromatography on a column of cation exchange resin eluted with a phosphate buffer with a gradient of NaCl and collecting and lyophilizing the active fraction.
Dependencies are inferred from the claim text.