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AssigneeTeijin Limited
InventorsKatsuhiko Tomibe, Yasuhiko Masuho, Kimihiko Matsuzawa, Sachio Ishimoto, Kazuo Satake, Tsuneo Watanabe
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Novel immunoglobulin derivatives which are characterized in that in which the interchain disulfide bonds of immunoglobulin are predominantly cleaved, on the average 3 to 5 of the interchain disulfide bonds or the inter- and intra-chain disulfide bonds being cleaved, and so produced sulfur atoms (S-) are S-sulfonated (--S-SO.sub.3). The above immunoglobulin derivatives are prepared by reacting native immunoglobulin with A. a compound capable of forming tetrathionate ion, and B. a compound capable of forming in water, sulfite ion whereby cleaving on the average 3 to 5 inter-chain disulfide bonds, or inter- and intra-chain disulfide bonds, of the native immunoglobulin, and S-sulfonating (S-SO.sub.3) the so formed sulfur atoms. This invention also provides a water-soluble composition for injection containing said immunoglobulin derivative having the titre of anti-diphtheria of at least 1.0 I.U./ml at its concentration of 10.0% by weight, and a pharmaceutically-acceptable solubilizing agent for said immunoglobulin derivative.
Novel immunoglobulin derivatives which are substantially free from oxidizing metal ions such as cupric ion comprising immunoglobulin in which the interchain disulfide bonds are predominantly cleaved, on the average 3 to 5 of the interchain disulfide bonds or the inter- and intra-chain disulfide bonds being cleaved, and so produced sulfur atoms (S--) are S-sulfonated (-S-SO.sub.3).
Immunoglobulin which is characterized in that
A water-soluble composition which comprises an immunoglobulin derivative which is substantially free from oxidizing metal ions in which on the average 3 to 5 interchain disulfide bonds, or inter- or intra-chain disulfide bonds, of native immunoglobulin are cleaved, and so formed sulfur atoms are S-sulfonated (S-SO.sub.3), and which exhibits the anti-diphtheria titre of at least 1.0 I.U./ml at the concentration of 10.0% by weight, and a harmless solubilizing agent of said immunoglobulin derivative.
A process for the preparation of immunoglobulin derivatives which comprises reacting native immunoglobulin with
Dependencies are inferred from the claim text.