Diluents for rubella virus hemagglutination-inhibition test
AssigneeTakeda Chemical Industries, Ltd.
InventorsSusumu Iwasa, Isamu Yoshida
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Abstract
In the rubella virus hemagglutination-inhibition test, the hemagglutination-inhibition antibody titers of the test sera can be determined with accuracy and high sensitivity by employing a novel diluent, which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a low concentration and which is free from calcium ion, for the dilution of the rubella virus hemagglutinating antigen and of the test sera.
Claims16
A diluent for rubella virus hemagglutinating antigen and for rubella virus hemagglutination-inhibition test sera, which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5, and which is free from calcium ion.
A method for rubella virus hemagglutination -inhibition test, which comprises admixing a test serum previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.001 to about 0.01 and NaCl, the pH of which is in the range of about 6.0 to about 7.5 and which is free from calcium ion with rubella virus hemagglutinating antigen previously diluted with the said diluent and, then, admixing the resultant mixture with a suspension of erythrocytes previously diluted with a diluent which contains N-2-hydroxyethylpiperazine-N'-2'-ethanesulfonic acid in a molar concentration from about 0.01 to about 0.05, CaCl.sub.2 in a molar concentration from about 0.0005 to about 0.01 and NaCl and the pH of which is in the range of about 6.0 to about 6.5 to assess agglutination or nonagglutination of the erythrocytes.
Dependencies are inferred from the claim text.